Orthogonal Strategies: Immunohistochemistry-Paraffin: Fatty Acid Synthase/FASN Antibody [NBP1-84733] - Staining in human breast and skeletal muscle tissues using anti-FASN antibody. Corresponding FASN RNA-seq ...read more
Independent Antibodies: Western Blot: Fatty Acid Synthase/FASN Antibody [NBP1-84733] - Analysis using Anti-FASN antibody NBP1-84733 (A) shows similar pattern to independent antibody NBP2-49213 (B).
Western Blot: Fatty Acid Synthase/FASN Antibody [NBP1-84733] - Analysis in human cell line SK-BR-3.
Immunocytochemistry/ Immunofluorescence: Fatty Acid Synthase/FASN Antibody [NBP1-84733] - Staining of human cell line A-431 shows positivity in plasma membrane & cytoplasm.
Immunohistochemistry-Paraffin: Fatty Acid Synthase/FASN Antibody [NBP1-84733] - Staining of human breast shows strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: Fatty Acid Synthase/FASN Antibody [NBP1-84733] - Staining of human skeletal muscle shows low expression as expected.
This antibody was developed against Recombinant Protein corresponding to amino acids: RWLRVTVAGGVHISGLHTESAPRRQQEQQVPILEKFCFTPHTEEGCLSERAALQEELQLCKGLVQALQTKVTQQGLKMVVPGLDGAQIPRDPSQQELPRLLSAACRLQLNGN
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FASN
Purity
Immunogen affinity purified
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WB reported in scientific literature (PMID: 22951905). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified
Alternate Names for Fatty Acid Synthase/FASN Antibody
EC 2.3.1
EC 2.3.1.85
EC 6.3.3.1
FASN
FASshort chain dehydrogenase/reductase family 27X, member 1
Fatty Acid Synthase
MGC14367
MGC15706
OA-519
SDR27X1
Background
Fatty Acid Synthase (FAS) is a very large enzymatic system involved in de novo lipogenesis (triglyceride production). Specifically, FAS catalyzes the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. It is also one of the accepted markers for insulin resistance and SREBP, LXR activation.
In some cancer cell lines, FAS has been found to be fused with ER-alpha. Therefore, FAS antibodies are useful tools for studies on fatty acid synthesis and research on certian cancers.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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