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Recombinant Human Fascin His Protein

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SDS-Page: Recombinant Human Fascin Protein [NBP1-45313] - 15% SDS-PAGE (3ug)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
1 mg/ml

Order Details

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Recombinant Human Fascin His Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-493 of Human Fascin

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MTANGTAEAV QIQFGLINCG NKYLTAEAFG FKVNASASSL KKKQIWTLEQ PPDEAGSAAV CLRSHLGRYL AADKDGNVTC EREVPGPDCR FLIVAHDDGR WSLQSEAHRR YFGGTEDRLS CFAQTVSPAE KWSVHIAMHP QVNIYSVTRK RYAHLSARPA DEIAVDRDVP WGVDSLITLA FQDQRYSVQT ADHRFLRHDG RLVARPEPAT GYTLEFRSGK VAFRDCEGRY LAPSGPSGTL KAGKATKVGK DELFALEQSC AQVVLQAANE RNVSTRQGMD LSANQDEETD QETFQLEIDR DTKKCAFRTH TGKYWTLTAT GGVQSTASSK NASCYFDIEW RDRRITLRAS NGKFVTSKKN GQLAASVETA GDSELFLMKL INRPIIVFRG EHGFIGCRKV TGTLDANRSS YDVFQLEFND GAYNIKDSTG KYWTVGSDSA VTSSGDTPVD FFFEFCDYNK VAIKVGGRYL KGDHAGVLKA SAETVDPASL WEY

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
FSCN1
Purity
>95%, by SDS-PAGE

Applications/Dilutions

Dilutions
  • SDS-Page
Theoretical MW
56.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP1-45313.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH8.0), 20% glycerol, 2 mM DTT, 100 mM NaCl
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>95%, by SDS-PAGE

Alternate Names for Recombinant Human Fascin His Protein

  • FAN1
  • FAN1,55 kDa actin-bundling protein
  • fascin homolog 1, actin-bundling protein (Strongylocentrotus purpuratus)
  • Fascin
  • FLJ38511
  • FSCN1
  • HSN
  • p55actin bundling protein
  • singed (Drosophila)-like (sea urchin fascin homolog like)
  • singed-like (fascin homolog, sea urchin)
  • Singed-like Protein
  • SNL
  • SNLfascin

Background

FSCN1 is an actin-bundling protein that provides rigidity to filopodial bundles to efficiently push the membrane forward during cytoskeleton remodeling and cell migration. Probably involved in the assembly of actin filament bundles present in microspikes, membrane ruffles, and stress fibers. And this protein is absent from most normal epithelia yet is upregulated in multiple forms of human carcinoma, where its expression correlates clinically with a poor prognosis. FSCN1 expression has been localized to neurons, glial cells, and endothelial cells. Recombinant human FSCN1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Publications for Fascin Recombinant Protein (NBP1-45313)(1)

Reviews for Fascin Recombinant Protein (NBP1-45313) (0)

There are no reviews for Fascin Recombinant Protein (NBP1-45313). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
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FAQs for Fascin Recombinant Protein (NBP1-45313). (Showing 1 - 1 of 1 FAQ).

  1. We are interested in ordering Novus' Fascin recombinant protein, catalog number: NBP1-45313 provided at 1.0 mg/mL. Has any testing been performed on the Fascin protein studying formation of the bundling of actin filaments by visualizing the actin bundles under a fluorescent microscope?
    • Thus far Novus Biologicals has only validated our protein for use in a SDS-PAGE application. We have not yet performed Immunofluorescence to visualize whether the Fascin protein forms the bundling of actin filaments. If you are willing to test the recombinant protein for visualization of this process we would encourage you to apply for our Innovators Reward Program. Under the terms of this program, you will be eligible for a full credit in exchange for your data, in the form of an online review, regardless of positive or negative results.

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Bioinformatics

Gene Symbol FSCN1