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Factor XII Antibody

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Western Blot: Factor XII/F12 Antibody [NBP1-94203] - WB analysis of F12 in 1. NIH 3T3 cell lysate and 2. Cos7 cell lysate.
Western Blot: Factor XII/F12 Antibody [NBP1-94203] - WB analysis of F12 in 1. NIH 3T3 cell lysate and 2. Cos7 cell lysate.
Immunocytochemistry/ Immunofluorescence: Factor XII Antibody [NBP1-94203] - F12 antibody was tested in NIH/3T3 cells with FITC (green). Nuclei were counterstained with DAPI (blue).
Immunohistochemistry: Factor XII Antibody [NBP1-94203] - IHC analysis of F12 in human renal cancer using DAB with hematoxylin counterstain.

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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Factor XII Antibody Summary

Immunogen
A synthetic peptide made to an internal portion of the human F12 protein (between residues 50-150) [UniProt P00748]
Localization
Secreted. Staining is also seen in the nucleus, not the nucleoli.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
F12
Purity
Unpurified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:400
  • Immunohistochemistry-Paraffin 1:400
  • Western Blot 1:1000
Application Notes
This Factor XII/F12 antibody is useful for Western Blot, Immunocytochemistry/Immunofluorescence, and IHC-paraffin embedded sections. In Western Blot, bands are seen ~67, 50, and 140kDa representing the full product, a clevage chain product, and a possible dimer. In ICC/IF, cytoplasmic staining was observed in NIH/3T3 cells. In IHC-P, staining was observed in the cytoplasm and nucleus of human renal cancer tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Control
NIH 3T3 Whole Cell Lysate

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for Factor XII Antibody

  • beta-factor XIIa part 1
  • coagulation factor XII (Hageman factor)
  • coagulation factor XII
  • coagulation factor XIIa heavy chain
  • coagulation factor XIIa light chain
  • EC 3.4.21
  • EC 3.4.21.38
  • F12
  • HAE3
  • HAEX
  • HAF beta-factor XIIa part 2
  • HAF
  • Hageman factor

Background

Coagulation factor XII (F12, Hageman factor, HAF, HAE3, HAEX), is a serum glycoprotein that is part of the peptidase S1 family. It is involved in hemostasis, blood coagulation, fibrinolysis, and the production of bradykinin and angiotensin. F12 is a secreted protein that can serve as a hydrolase, protease, and serine protease. F12 is triggered by kallikrein in alpha-factor XIIa, which is then further altered by trypsin into beta-factor XIIa. F12 has one known isoform, and the protein contains two EGF-like domains, a fibronectin type-I domain, a fibronectin type-II domain, a Kringle domain, and a peptidase S1 domain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Control Lysate(s)

NIH 3T3 Whole Cell Lysate

Secondary Antibodies

 

Isotype Controls

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Product PDFs

  ICC/IF Protocol
  IHC Protocol
  Western Blot Protocol
  SDS
  PDF Datasheet
  Request CofA

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Bioinformatics

Gene Symbol F12
Entrez
Uniprot