EVI-1 Antibody

Images

 
Western Blot: Goat Polyclonal EVI-1 Antibody [NBP1-68842] - (3µg/ml) staining of A431 (A), Caco-2 (B) and HeLa (C) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

EVI-1 Antibody Summary

Immunogen
EVI1 / AML1, internal region
Epitope
C-DKESLHSTSH
Specificity
This antibody is expected to recognize all reported isoforms (NP_001098547.3; NP_005232.2; NP_004982.2; NP_001157471.1; NP_001157472.1). Reported variants represent identical protein: NP_001098548.2, NP_005232.2
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MECOM
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:16000
  • Western Blot 1-3 ug/ml
Application Notes
Approx. 140-150kDa band observed in lysates of cell lines A431, Caco-2 and HeLa (calculated MW of 126kDa according to NP_001098547.3). This molecular weight is routinely observed by other commercial sources.
Publications
Read Publication using NBP1-68842.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for EVI-1 Antibody

  • AML1-EVI-1 fusion protein
  • AML1-EVI-1
  • ecotropic viral integration site 1
  • Ecotropic virus integration site 1 protein homolog
  • EVI1
  • EVI-1
  • EVI1MDS1-EVI1
  • MDS1 and EVI1 complex locus protein EVI1
  • MDS1 and EVI1 complex locus protein MDS1
  • MDS1 and EVI1 complex locus
  • MDS1
  • MECOM
  • MGC163392
  • MGC97004
  • Myelodysplasia syndrome 1 protein
  • myelodysplasia syndrome 1
  • Myelodysplasia syndrome-associated protein 1
  • oncogene EVI1
  • PRDM3
  • zinc finger protein Evi1

Background

EVI1 promotes cell proliferation by interacting with BRG1 and blocking the repression of BRG1 on E2F1 activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for EVI-1 Antibody (NBP1-68842)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MECOM
Entrez
Uniprot