![Western Blot: Ribophorin I Antibody (OTI3H3) [NBP2-01294] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Ribophorin I (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Ribophorin I.](http://images.novusbio.com/fullsize/Ribophorin-I-Antibody-OTI3H3-Western-Blot-NBP2-01294-img0016.jpg "Western Blot: Ribophorin I Antibody (OTI3H3) [NBP2-01294] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Ribophorin I (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Ribophorin I.")
| Reactivity | Hu, Mu, Rt, Ca, Pm, PmSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC |
| Clone | OTI3H3 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Full length human recombinant protein of human RPN1(NP_002941) produced in HEK293T cell. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | RPN1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
|
| Theoretical MW | 66.2 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
|
| Reviewed Applications |
|
|
| Publications |
|
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 1.0% BSA and 50% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Immunogen affinity purified |
![Immunocytochemistry CCR7 Antibody (150503) [Unconjugated]](https://images.novusbio.com/images2/CCR7_MAB197_Immunocytochemistry_6580.jpg)
![Immunohistochemistry CCR7 Antibody (150503) [Unconjugated]](https://images.novusbio.com/images2/CCR7_MAB197_Immunohistochemistry_22800.jpg)
![Neutralization CCR7 Antibody (150503) [Unconjugated]](https://images.novusbio.com/images2/CCR7_MAB197_Block_Neutralize_9048.jpg)
| Publication using NBP2-01294 | Applications | Species |
|---|---|---|
| Seo JS, Min BS, Kim YJ et al. Effect of glucose feeding on the glycosylation quality of antibody produced by a human cell line, F2N78, in fed-batch culture. Appl Microbiol Biotechnol 2014-01-03 [PMID: 24384750] (WB, Human) | WB | Human |
| Images | Ratings | Applications | Species | Date | Details | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Enlarge |
reviewed by:
Teddy Chan |
WB | Human | 02/08/2019 |
Summary
Comments
|
||||||||||
Enlarge |
reviewed by:
Verified Customer |
ICC | Human | 07/01/2014 |
Summary
|
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
5 | |
4 | |
3 | |
2 | |
1 |
| Teddy Chan 02/08/2019 |
||
| Application: | WB | |
| Species: | Human |
| Verified Customer 07/01/2014 |
||
| Application: | ICC | |
| Species: | Human |
![Immunocytochemistry/Immunofluorescence: Ribophorin I Antibody (OTI3H3) [NBP2-01294] - expression of RPN1 on pca cell lines vs normal. Image from verified customer review.](http://images.novusbio.com/fullsize/Ribophorin-I-Antibody-OTI3H3-Immunocytochemistry-Immunofluorescence-NBP2-01294-img0010.jpg "Immunocytochemistry/Immunofluorescence: Ribophorin I Antibody (OTI3H3) [NBP2-01294] - expression of RPN1 on pca cell lines vs normal. Image from verified customer review.")
![Immunohistochemistry: Ribophorin I Antibody (OTI3H3) [NBP2-01294] - Staining of paraffin-embedded Human pancreas tissue using anti-Ribophorin I mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Ribophorin-I-Antibody-OTI3H3-Immunohistochemistry-NBP2-01294-img0018.jpg "Immunohistochemistry: Ribophorin I Antibody (OTI3H3) [NBP2-01294] - Staining of paraffin-embedded Human pancreas tissue using anti-Ribophorin I mouse monoclonal antibody.")
![Flow Cytometry: Ribophorin I Antibody (OTI3H3) [NBP2-01294] - HEK293T cells transfected with either Ribophorin I Human Tagged ORF Clone overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RPN1 antibody , and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Ribophorin-I-Antibody-OTI3H3-Flow-Cytometry-NBP2-01294-img0019.jpg "Flow Cytometry: Ribophorin I Antibody (OTI3H3) [NBP2-01294] - HEK293T cells transfected with either Ribophorin I Human Tagged ORF Clone overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RPN1 antibody , and then analyzed by flow cytometry.")
![Western Blot: Ribophorin I Antibody (OTI3H3) [NBP2-01294] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Ribophorin I monoclonal antibody.](http://images.novusbio.com/fullsize/Ribophorin-I-Antibody-OTI3H3-Western-Blot-NBP2-01294-img0009.jpg "Western Blot: Ribophorin I Antibody (OTI3H3) [NBP2-01294] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Ribophorin I monoclonal antibody.")
![Immunohistochemistry-Paraffin: Ribophorin I Antibody (OTI3H3) [NBP2-01294] - Staining of paraffin-embedded Human endometrium tissue using anti-Ribophorin I mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Ribophorin-I-Antibody-OTI3H3-Immunohistochemistry-Paraffin-NBP2-01294-img0006.jpg "Immunohistochemistry-Paraffin: Ribophorin I Antibody (OTI3H3) [NBP2-01294] - Staining of paraffin-embedded Human endometrium tissue using anti-Ribophorin I mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: Ribophorin I Antibody (OTI3H3) [NBP2-01294] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Ribophorin I mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Ribophorin-I-Antibody-OTI3H3-Immunohistochemistry-Paraffin-NBP2-01294-img0011.jpg "Immunohistochemistry-Paraffin: Ribophorin I Antibody (OTI3H3) [NBP2-01294] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Ribophorin I mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: Ribophorin I Antibody (OTI3H3) [NBP2-01294] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-Ribophorin I mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Ribophorin-I-Antibody-OTI3H3-Immunohistochemistry-Paraffin-NBP2-01294-img0012.jpg "Immunohistochemistry-Paraffin: Ribophorin I Antibody (OTI3H3) [NBP2-01294] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-Ribophorin I mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: Ribophorin I Antibody (OTI3H3) [NBP2-01294] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-Ribophorin I mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Ribophorin-I-Antibody-OTI3H3-Immunohistochemistry-Paraffin-NBP2-01294-img0013.jpg "Immunohistochemistry-Paraffin: Ribophorin I Antibody (OTI3H3) [NBP2-01294] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-Ribophorin I mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: Ribophorin I Antibody (OTI3H3) [NBP2-01294] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-Ribophorin I mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Ribophorin-I-Antibody-OTI3H3-Immunohistochemistry-Paraffin-NBP2-01294-img0014.jpg "Immunohistochemistry-Paraffin: Ribophorin I Antibody (OTI3H3) [NBP2-01294] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-Ribophorin I mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: Ribophorin I Antibody (OTI3H3) [NBP2-01294] - Staining of paraffin-embedded Human liver tissue using anti-Ribophorin I mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Ribophorin-I-Antibody-OTI3H3-Immunohistochemistry-Paraffin-NBP2-01294-img0015.jpg "Immunohistochemistry-Paraffin: Ribophorin I Antibody (OTI3H3) [NBP2-01294] - Staining of paraffin-embedded Human liver tissue using anti-Ribophorin I mouse monoclonal antibody.")
![Western Blot PRDM16/MEL1 Antibody [Unconjugated]](https://images.novusbio.com/images2/PRDM16_AF6295_Western_Blot_10251.jpg)
![Immunohistochemistry PRDM16/MEL1 Antibody [Unconjugated]](https://images.novusbio.com/images2/PRDM16_AF6295_Immunohistochemistry_10448.jpg)
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")
