CD34 Antibody (MEC 14.7)

CD34, also known as Cluster of Differentiation 34, is a transmembrane phosphoglycoprotein that is primarily known for being a surface marker for hematopoietic stem cells (HSCs) and progenitor cells (1,2). The CD34 protein consists of a heavily glycosylated extracellular domain, a single-pass transmembrane helix, and a cytoplasmic tail with PDZ binding motifs (1,3). Human CD34 has two protein isoforms consisting of 385 amino acids (aa) for the canonical isoform and 328 aa for isoform 2 (3,4). The canonical CD34 isoform has calculated theoretical molecular weight of 40 kDa and an observed molecular weight of ~115 kDa (1,3,4). L-Selectin (CD62L) is the most common ligand for CD34, but it has also been shown to bind CrkL (1,3).

CD34 has commonly been used as a marker for the diagnosis and classification of various diseases and pathologies including leukemia and solitary fibrous tumor (SFT) (2,5). In terms of immunohistochemistry and histopathology, CD34 has been the most common marker for SFT and is expressed in ~79% of cases (5). In addition to its use as a cell marker, CD34-postive (CD34+) hematopoietic stem cells have been used therapeutically in patients following radiation or chemotherapy due to their regenerative potential (6). There are several clinical trials showing promising results for CD34+ cell therapy for cardiovascular diseases including heart failure, ischemia, dilated cardiomyopathy, acute myocardial infarction, and angina (6). Besides hematopoietic lineages, CD34 is also expressed in non-hematopoietic cells including mesenchymal stem cells, endothelial cells and progenitors, fibrocytes, muscle satellite cells, and some cancer stem cells (1,3). While the clinical and cell therapy applications of CD34 as a cell marker is well documented, the function of CD34 is less understood but has been implicated in many cellular processes such as adhesion, proliferation, signal transduction, differentiation, and progenitor phenotype maintenance (1,3).

References

1. Sidney, L. E., Branch, M. J., Dunphy, S. E., Dua, H. S., & Hopkinson, A. (2014). Concise review: evidence for CD34 as a common marker for diverse progenitors. Stem cells (Dayton, Ohio), 32(6), 1380-1389. https://doi.org/10.1002/stem.1661

2. Krause, D. S., Fackler, M. J., Civin, C. I., & May, W. S. (1996). CD34: structure, biology, and clinical utility. Blood, 87(1), 1-13

3. Kapoor, S., Shenoy, S. P., & Bose, B. (2020). CD34 cells in somatic, regenerative and cancer stem cells: Developmental biology, cell therapy, and omics big data perspective. Journal of cellular biochemistry, 121(5-6), 3058-3069. https://doi.org/10.1002/jcb.29571

4. Uniprot (P28906)

5. Davanzo, B., Emerson, R. E., Lisy, M., Koniaris, L. G., & Kays, J. K. (2018). Solitary fibrous tumor. Translational gastroenterology and hepatology, 3, 94. https://doi.org/10.21037/tgh.2018.11.02

6. Prasad, M., Corban, M. T., Henry, T. D., Dietz, A. B., Lerman, L. O., & Lerman, A. (2020). Promise of autologous CD34+ stem/progenitor cell therapy for treatment of cardiovascular disease. Cardiovascular research, 116(8), 1424-1433. https://doi.org/10.1093/cvr/cvaa027

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Rabbit.

We have publications tested in 7 applications: FLOW, Flow, ICC/IF, IF/IHC, IHC-Fr, IHC-P, WB.

Showing Publications 1 - 10 of 28. Show All 28 Publications. Collapse Publications.

Publications using NB600-1071	Applications	Species
Celikten M, Sahin H, Senturk GE et al. The Effect of Platelet-Rich Fibrin, Platelet-Rich Plasma, and Concentrated Growth Factor in the Repair of Full Thickness Rotator Cuff Tears Journal of shoulder and elbow surgery 2023-10-26 [PMID: 37898418] (IHC-P, Rabbit)	IHC-P	Rabbit
Liu Y, Zhou Y. Circ_0087960 stabilizes KDM5B by reducing SKP2 mediated ubiquitination degradation and promotes osteogenic differentiation in periodontal ligament stem cells Regen Ther 2022-03-01 [PMID: 35229010] (FLOW, Human) Details: Citation using the FITC version of this antibody.	FLOW	Human
Fjaestad KY, Romer AMA, Goitea V et al. Blockade of beta-adrenergic receptors reduces cancer growth and enhances the response to anti-CTLA4 therapy by modulating the tumor microenvironment Oncogene 2022-01-11 [PMID: 35017664] (IHC-P, Mouse)	IHC-P	Mouse
Wang T, Bai J, Lu M et al. Engineering immunomodulatory and osteoinductive implant surfaces via mussel adhesion-mediated ion coordination and molecular clicking Nature Communications 2022-01-10 [PMID: 35013289] (FLOW)	FLOW
Lynch Em, Robertson S, Fitzgibbons C Et Al. Transcriptome analysis using patient iPSC-derived skeletal myocytes: Bet1L as a new molecule possibly linked to neuromuscular degeneRation in ALS Experimental neurology 2021-07-23 [PMID: 34310943]
Costa B, Fletcher MNC, Boskovic P, et al. A Set of Cell Lines Derived from a Genetic Murine Glioblastoma Model Recapitulates Molecular and Morphological Characteristics of Human Tumors Cancers 2021-01-10 [PMID: 33435218] (IHC-P, Mouse)	IHC-P	Mouse
Zhou C, Zhou L, Liu J et al. Kidney extracellular matrix hydrogel enhances therapeutic potential of adipose-derived mesenchymal stem cells for renal ischemia reperfusion injury Acta Biomater 2020-08-06 [PMID: 32771597]
Kolb R, Kluz P, Tan ZW et al. Obesity-associated inflammation promotes angiogenesis and breast cancer via angiopoietin-like 4 Oncogene 2018-12-05 [PMID: 30518876] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Sakurai R, Liu J, Wang Y et al. Prevention of perinatal nicotine-induced bone marrow mesenchymal stem cell myofibroblast differentiation by augmenting the lipofibroblast phenotype Clin Sci (Lond) 4338-01-01 [PMID: 30309879] (FLOW)	FLOW
Braun RK, Chetty C, Balasubramaniam V, et al Intraperitoneal injection of MSC-derived exosomes prevent experimental bronchopulmonary dysplasia. Biochem Biophys Res Commun. 2018 Aug 07 [PMID: 30093115] (Flow, Rat)	Flow	Rat
Xu B, Li Y, Deng B et al. Chitosan hydrogel improves mesenchymal stem cell transplant survival and cardiac function following myocardial infarction in rats. Exp Ther Med. Feb 1 2017 [PMID: 28352335] (ICC/IF, Rat)	ICC/IF	Rat
Wei L, Wang H, Yang F et al. Interleukin-17 potently increases non-small cell lung cancer growth. Mol Med Rep. 2016-02-01 [PMID: 26708832] Details: This citation used the HRP version of this antibody.
Biktasova AK, Dudimah DF, Uzhachenko RV et al. Multivalent forms of the Notch ligand DLL-1 enhance antitumor T cell immunity in lung cancer and improve efficacy of EGFR targeted therapy. Cancer Res. 2015-09-24 [PMID: 26404003] (IHC-P, Mouse)	IHC-P	Mouse
Borriello L, Montes M, Lepelletier Y et al. Structure-based discovery of a small non-peptidic Neuropilins antagonist exerting in vitro and in vivo anti-tumor activity on breast cancer model. Cancer Lett 2014-07-28 [PMID: 24752068] (IHC-Fr, Mouse) Details: Using the DyLight 488 conjugated version of NB600-1071, catalog number NB600-1071G.	IHC-Fr	Mouse
Scheerer N, Dehne N, Stockmann C et al. Myeloid hypoxia-inducible factor-1alpha is essential for skeletal muscle regeneration in mice. J Immunol 2013-07-01 [PMID: 23729446] (IHC-P, Mouse)	IHC-P	Mouse
Savi? R, He X, Fiel I, Schuchman EH. Recombinant Human Acid Sphingomyelinase as an Adjuvant to Sorafenib Treatment of Experimental Liver Cancer. PLoS One 2013-05-28 [PMID: 23724146] (IHC-P, Mouse)	IHC-P	Mouse
Lee YS, Morinaga H, Kim JJ et al. The Fractalkine/CX3CR1 System Regulates beta Cell Function and Insulin Secretion. Cell 2013-04-11 [PMID: 23582329] (IHC-P, Mouse)	IHC-P	Mouse
Huang YS, Li IH, Chueh SH et al. Mesenchymal stem cells from rat olfactory bulbs can differentiate into cells with cardiomyocyte characteristics. J Tissue Eng Regen Med 2013-02-04 [PMID: 23378029]
Branco-Price C, Zhang N, Schnelle M et al. Endothelial Cell HIF-1[alpha] and HIF-2[alpha] Differentially Regulate Metastatic Success. Cancer Cell 1721(1):52-65. 2012-01-01 [PMID: 22264788]
Fiorina P, Pietramaggiori G, Scherer SS et al. The mobilization and effect of endogenous bone marrow progenitor cells in diabetic wound healing. Cell Transplant;19(11):1369-81. 2010-01-01 [PMID: 20977829]
Sorensen EM Plasma-derived exosomes as potential biomarkers for pediatric acute leukemia. Thesis 2020-01-01 (WB, Human) Details: Exosomes from human plasma samples underwent Western blot analysis.	WB	Human
Kalabis J, Oyama K, Okawa T, Nakagawa H, Michaylira CZ, Stairs DB, Figueiredo JL, Mahmood U, Diehl JA, Herlyn M, Rustgi AK. A subpopulation of mouse esophageal basal cells has properties of stem cells with the capacity for self-renewal and lineage specification. J Clin Invest;118(12):3860-9. 2008-12-01 [PMID: 19033657] (ICC/IF, IHC-P, FLOW, Mouse)	ICC/IF, IHC-P, FLOW	Mouse
Sironi M, Conti A, Bernasconi S, Fra AM, Pasqualini F, Nebuloni M, Lauri E, De Bortoli M, Mantovani A, Dejana E, Vecchi A. Generation and characterization of a mouse lymphatic endothelial cell line. Cell Tissue Res;325(1):91-100. 2006-07-01 [PMID: 16534603]
Zheng Y, Du X, Wang W, Boucher M, Parimoo S, Stenn K. Organogenesis from dissociated cells: generation of mature cycling hair follicles from skin-derived cells. J Invest Dermatol;124(5):867-76. 2005-05-01 [PMID: 15854024] (IHC-P, Mouse)	IHC-P	Mouse
Trempus CS et al. Enrichment for living murine keratinocytes from the hair follicle bulge with the cell surface marker CD34. J Invest Dermatol 120:501-11. 2003-01-01 [PMID: 12648211] (FLOW, IF/IHC, ICC/IF)	FLOW, IF/IHC, ICC/IF
Vecchi A, Massimiliano L, Ramponi S, Luini W, Bernasconi S, Bonecchi R, Allavena P, Parmentier M, Mantovani A, Sozzani S. Differential responsiveness to constitutive vs inducible chemokines of immature and mature mouse dendritic cells. J Leukoc Biol;66(3):489-94. 1999-09-01 [PMID: 10496320]
Dong QG, Bernasconi S, Lostaglio S et al. A general strategy for isolation of endothelial cells from murine tissues. Characterization of two endothelial cell lines from the murine lung and subcutaneous sponge implants. Arterioscler Thromb Vasc Biol. 1997-08-01 [PMID: 9301641] (FLOW, Mouse)	FLOW	Mouse
Garlanda C, Berthier R, Garin J et al. Characterization of MEC 14.7, a new monoclonal antibody recognizing mouse CD34: a useful reage for identifying and characterizing blood vessels and hematopoietic precursors. Eur J Cell Biol. 1997-08-01 [PMID: 9270880]
Show All 28 Publications. Collapse Publications.

Array NB600-1071

• CD34 Antibodies
• CD34 Antibody Pair
• CD34 ELISA Kit
• CD34 Lysates
• CD34 Proteins
• CD34 Proteins and Enzymes