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ERK3/MAPK6 Antibody (OTI5E1) - Azide and BSA Free

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Western Blot: ERK3/MAPK6 Antibody (OTI5E1) - Azide and BSA Free [NBP2-71242] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK6(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK6.
Western Blot: ERK3/MAPK6 Antibody (OTI5E1) - Azide and BSA Free [NBP2-71242] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK6(Right lane) cDNA for 48 hrs and lysed. ...read more
Immunocytochemistry/ Immunofluorescence: ERK3/MAPK6 Antibody (OTI5E1) - Azide and BSA Free [NBP2-71242] - Staining of HeLa cells transiently transfected by pCMV6-ENTRY MAPK6.
Immunohistochemistry: ERK3/MAPK6 Antibody (OTI5E1) - Azide and BSA Free [NBP2-71242] - Staining of HeLa cells using anti-MAPK6 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
OTI5E1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-71242AF350
Alexa Fluor 405
NBP2-71242AF405
Alexa Fluor 488
NBP2-71242AF488
Alexa Fluor 532
NBP2-71242AF532
Alexa Fluor 594
NBP2-71242AF594
Alexa Fluor 647
NBP2-71242AF647
Alexa Fluor 700
NBP2-71242AF700
Alexa Fluor 750
NBP2-71242AF750
Allophycocyanin
NBP2-71242APC
Biotin
NBP2-71242B
CoraFluor 1
NBP2-71242CL1
DyLight 350
NBP2-71242UV
DyLight 405
NBP2-71242V
DyLight 488
NBP2-71242G
DyLight 550
NBP2-71242R
DyLight 594
NBP2-71242DL594
DyLight 650
NBP2-71242C
DyLight 680
NBP2-71242FR
DyLight 755
NBP2-71242IR
FITC
NBP2-71242F
HRP
NBP2-71242H
Janelia Fluor 525
NBP2-71242JF525
Janelia Fluor 549
NBP2-71242JF549
Janelia Fluor 646
NBP2-71242JF646
Janelia Fluor 669
NBP2-71242JF669
PE
NBP2-71242PE
PerCP
NBP2-71242PCP
Unconjugated
NBP1-47854
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP1-47854
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ERK3/MAPK6 Antibody (OTI5E1) - Azide and BSA Free Summary

Immunogen
Recombinant protein expressed in E.coli corresponding to amino acids 345-721 of human ERK3
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
MAPK6
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:1000
Theoretical MW
82.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for ERK3/MAPK6 Antibody (OTI5E1) - Azide and BSA Free

  • DKFZp686F03189
  • EC 2.7.11
  • EC 2.7.11.24
  • ERK3
  • ERK-3
  • ERK3p97-MAPK
  • Extracellular signal-regulated kinase 3
  • extracellular signal-regulated kinase, p97
  • HsT17250
  • MAP kinase 6
  • MAP kinase isoform p97
  • MAPK 6
  • MAPK6
  • mitogen-activated protein kinase 6
  • p97MAPK
  • PRKM6
  • protein kinase, mitogen-activated 5
  • protein kinase, mitogen-activated 6

Background

ERK3 (Extracellular signal-regulated kinase 3, MAPK6, p97 MAPK) belongs to the Ser/Thr protein kinase family and is a member of the mitogen-activated protein (MAP) kinase superfamily. Unlike the other members of the MAPK family, ERK3 is a mitogen-activated protein kinase homologue that has SEG motif instead of conserved TXY motif in the activation loop (1). Also, unlike ERK1 and ERK2, it is ubiquitously expressed and localized in the nucleus of proliferating cells (2). Highly unstable, ERK3 is constitutively degraded during ubiquitin-proteasome pathway of proliferating cells (3). ERK3 has been linked to phosphorylation MAP2 and as a possible regulator during cell differentiation (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ERK3/MAPK6
Antibody (OTI5E1) - A ...
NBP2-71242
ERK3/MAPK6 Antibody (OTI5E1) - Azide and BSA Free
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC

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Bioinformatics

Gene Symbol MAPK6