| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB3196-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71242 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3196-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-03701-20ul | |||
![Western Blot: ERK3/MAPK6 Antibody (5E1) [NBP1-47854] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK6(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK6.](http://images.novusbio.com/fullsize/ERK3-MAPK6-Antibody-5E1-Western-Blot-NBP1-47854-img0006.jpg "Western Blot: ERK3/MAPK6 Antibody (5E1) [NBP1-47854] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK6(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK6.")
![Immunocytochemistry/Immunofluorescence: ERK3/MAPK6 Antibody (5E1) [NBP1-47854] - Staining of HeLa cells transiently transfected by pCMV6-ENTRY MAPK6.](http://images.novusbio.com/fullsize/ERK3-MAPK6-Antibody-5E1-Immunocytochemistry-Immunofluorescence-NBP1-47854-img0004.jpg)
![Immunohistochemistry-Paraffin: ERK3/MAPK6 Antibody (5E1) [NBP1-47854] - Staining of HeLa cells using anti-MAPK6 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ERK3-MAPK6-Antibody-5E1-Immunohistochemistry-Paraffin-NBP1-47854-img0005.jpg)
![Western Blot: ERK3/MAPK6 Antibody (1G6) [H00005597-M01] - Analysis of MAPK6 expression in transfected 293T cell line by MAPK6 monoclonal antibody (M01), clone 1G6.Lane 1: MAPK6 transfected lysate(82.7 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/ERK3-MAPK6-Antibody-1G6-Western-Blot-H00005597-M01-img0008.jpg "Western Blot: ERK3/MAPK6 Antibody (1G6) [H00005597-M01] - Analysis of MAPK6 expression in transfected 293T cell line by MAPK6 monoclonal antibody (M01), clone 1G6.Lane 1: MAPK6 transfected lysate(82.7 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: ERK3/MAPK6 Antibody (1G6) [H00005597-M01] - Analysis of monoclonal antibody to MAPK6 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/ERK3-MAPK6-Antibody-1G6-Immunocytochemistry-Immunofluorescence-H00005597-M01-img0005.jpg)
![Western Blot: ERK3/MAPK6 Antibody (1G6) [H00005597-M01] - Analysis of MAPK6 over-expressed 293 cell line, cotransfected with MAPK6 Validated Chimera RNAi ( Cat # H00005597-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MAPK6 monoclonal antibody (M01) clone 1G6 (Cat # H00005597-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/ERK3-MAPK6-Antibody-1G6-Western-Blot-H00005597-M01-img0006.jpg)
![Sandwich ELISA: ERK3/MAPK6 Antibody (1G6) [H00005597-M01] - Detection limit for recombinant GST tagged MAPK6 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/ERK3-MAPK6-Antibody-1G6-Sandwich-ELISA-H00005597-M01-img0007.jpg)
![Western Blot: ERK3/MAPK6 Antibody [NBP1-84806] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/ERK3-MAPK6-Antibody-Western-Blot-NBP1-84806-img0004.jpg "Western Blot: ERK3/MAPK6 Antibody [NBP1-84806] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4")
![Immunocytochemistry/Immunofluorescence: ERK3/MAPK6 Antibody [NBP1-84806] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol.](http://images.novusbio.com/fullsize/ERK3-MAPK6-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84806-img0006.jpg)
![Immunohistochemistry-Paraffin: ERK3/MAPK6 Antibody [NBP1-84806] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/ERK3-MAPK6-Antibody-Immunohistochemistry-Paraffin-NBP1-84806-img0002.jpg)
![Western Blot: ERK3/MAPK6 Antibody (PSH03-39) [NBP3-32567] - Western blot analysis of ERK3/MAPK6 on different lysates with Rabbit anti-ERK3/MAPK6 antibody (NBP3-32567) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: A431 cell lysate Lane 3: A549 cell lysate Lane 4: PC-3M cell lysate Lane 5: C2C12 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: C6 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 83 kDa Observed band size: 105 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32567) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32567_rabbit-erk3-mapk6-mab-psh03-39-278202415212460.jpg "Western Blot: ERK3/MAPK6 Antibody (PSH03-39) [NBP3-32567] - Western blot analysis of ERK3/MAPK6 on different lysates with Rabbit anti-ERK3/MAPK6 antibody (NBP3-32567) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: A431 cell lysate Lane 3: A549 cell lysate Lane 4: PC-3M cell lysate Lane 5: C2C12 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: C6 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 83 kDa Observed band size: 105 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32567) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: ERK3/MAPK6 Antibody (PSH03-39) [NBP3-32567] - Immunocytochemistry analysis of 293T cells labeling ERK3/MAPK6 with Rabbit anti-ERK3/MAPK6 antibody (NBP3-32567) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ERK3/MAPK6 antibody (NBP3-32567) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32567_rabbit-erk3-mapk6-mab-psh03-39-2920241192388.jpg)
![Flow Cytometry: ERK3/MAPK6 Antibody (PSH03-39) [NBP3-32567] - Flow cytometric analysis of 293T cells labeling ERK3/MAPK6. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32567, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32567_rabbit-erk3-mapk6-mab-psh03-39-392024884681.jpg)
![Western Blot: ERK3/MAPK6 Antibody [NBP3-03701] - Analysis of extracts of various cell lines, using ERK3/MAPK6 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/ERK3-MAPK6-Antibody-Western-Blot-NBP3-03701-img0003.jpg "Western Blot: ERK3/MAPK6 Antibody [NBP3-03701] - Analysis of extracts of various cell lines, using ERK3/MAPK6 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
![Immunocytochemistry/Immunofluorescence: ERK3/MAPK6 Antibody [NBP3-03701] - Analysis of L929 cells using ERK3/MAPK6 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/ERK3-MAPK6-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-03701-img0001.jpg)
![Immunohistochemistry-Paraffin: ERK3/MAPK6 Antibody [NBP2-99003] - Immunochemical staining of human ERK3/MAPK6 in rat heart with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/ERK3-MAPK6-Antibody-Immunohistochemistry-Paraffin-NBP2-99003-img0003.jpg "Immunohistochemistry-Paraffin: ERK3/MAPK6 Antibody [NBP2-99003] - Immunochemical staining of human ERK3/MAPK6 in rat heart with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.")
![Immunohistochemistry-Paraffin: ERK3/MAPK6 Antibody [NBP2-99003] - Immunochemical staining of human ERK3/MAPK6 in cynomolgus heart with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/ERK3-MAPK6-Antibody-Immunohistochemistry-Paraffin-NBP2-99003-img0001.jpg)
![Immunohistochemistry-Paraffin: ERK3/MAPK6 Antibody [NBP2-99003] - Immunochemical staining of human ERK3/MAPK6 in mouse heart with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/ERK3-MAPK6-Antibody-Immunohistochemistry-Paraffin-NBP2-99003-img0002.jpg)
![ELISA: Human ERK3/MAPK6 ELISA Kit (Colorimetric) [NBP3-00379] - Samples were spiked with high concentrations of Human ERK3/MAPK6 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-00379_human-erk3-mapk6-elisa-kit-colorimetric-132202416211381.jpg "ELISA: Human ERK3/MAPK6 ELISA Kit (Colorimetric) [NBP3-00379] - Samples were spiked with high concentrations of Human ERK3/MAPK6 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human ERK3/MAPK6 ELISA Kit (Colorimetric) [NBP3-00379] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-00379_human-erk3-mapk6-elisa-kit-colorimetric-43202492402.jpg)
![Western Blot: MAPK6 Overexpression Lysate (Adult Normal) [NBL1-12878] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MAPK6.](http://images.novusbio.com/fullsize/MAPK6-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-12878-img0002.jpg "Western Blot: MAPK6 Overexpression Lysate (Adult Normal) [NBL1-12878] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MAPK6.")