Home » ERK3/MAPK6 » ERK3/MAPK6 Antibodies » ERK3/MAPK6 Antibody (OTI5E1)
Novus Biologicals products are now on bio-techne.com

ERK3/MAPK6 Antibody (OTI5E1)

 Submit a Review

Images

 
(
Enlarge)
Western Blot: ERK3/MAPK6 Antibody (5E1) [NBP1-47854] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK6(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK6.
Western Blot: ERK3/MAPK6 Antibody (5E1) [NBP1-47854] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK6(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell ...read more
Immunocytochemistry/ Immunofluorescence: ERK3/MAPK6 Antibody (5E1) [NBP1-47854] - Staining of HeLa cells transiently transfected by pCMV6-ENTRY MAPK6.
Immunohistochemistry-Paraffin: ERK3/MAPK6 Antibody (5E1) [NBP1-47854] - Staining of HeLa cells using anti-MAPK6 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
OTI5E1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-71242AF350
Alexa Fluor 405
NBP2-71242AF405
Alexa Fluor 488
NBP2-71242AF488
Alexa Fluor 532
NBP2-71242AF532
Alexa Fluor 594
NBP2-71242AF594
Alexa Fluor 647
NBP2-71242AF647
Alexa Fluor 700
NBP2-71242AF700
Alexa Fluor 750
NBP2-71242AF750
Allophycocyanin
NBP2-71242APC
Biotin
NBP2-71242B
DyLight 350
NBP2-71242UV
DyLight 405
NBP2-71242V
DyLight 488
NBP2-71242G
DyLight 550
NBP2-71242R
DyLight 594
NBP2-71242DL594
DyLight 650
NBP2-71242C
DyLight 680
NBP2-71242FR
DyLight 755
NBP2-71242IR
FITC
NBP2-71242F
HRP
NBP2-71242H
Janelia Fluor 549
NBP2-71242JF549
Janelia Fluor 646
NBP2-71242JF646
PE
NBP2-71242PE
PerCP
NBP2-71242PCP
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-71242
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

ERK3/MAPK6 Antibody (OTI5E1) Summary

Immunogen
Recombinant protein expressed in E.coli corresponding to amino acids 345-721 of human ERK3
Specificity
This antibody is specific for Homo sapiens mitogen-activated protein kinase 6 (MAPK6).
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
MAPK6
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:1000
Theoretical MW
82.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ERK3/MAPK6 Antibody (OTI5E1)

  • DKFZp686F03189
  • EC 2.7.11
  • EC 2.7.11.24
  • ERK3
  • ERK-3
  • ERK3p97-MAPK
  • Extracellular signal-regulated kinase 3
  • extracellular signal-regulated kinase, p97
  • HsT17250
  • MAP kinase 6
  • MAP kinase isoform p97
  • MAPK 6
  • MAPK6
  • mitogen-activated protein kinase 6
  • p97MAPK
  • PRKM6
  • protein kinase, mitogen-activated 5
  • protein kinase, mitogen-activated 6

Background

ERK3 is a member of the Ser/Thr protein kinase family, and is most closely related to mitogen-activated protein kinases (MAP kinases). MAP kinases also known as extracellular signal-regulated kinases (ERKs), are activated through protein phosphorylation cascades and act as integration points for multiple biochemical signals. This kinase is localized in the nucleus, and has been reported to be activated in fibroblasts upon treatment with serum or phorbol esters

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

ERK1 Antibody
(1E5) - BSA Free
NBP2-22203
Flow Cytometry ERK1 Antibody (1E5) - BSA Free
Immunocytochemistry/ Immunofluorescence ERK1 Antibody (1E5) - BSA Free
Immunohistochemistry-Paraffin ERK1 Antibody (1E5) - BSA Free
(1 Publication)
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
ERK2 Antibody
[Unconjugated]
AF1230
Western Blot ERK2 Antibody [Unconjugated]
Immunohistochemistry ERK2 Antibody [Unconjugated]
Knockout Validated ERK2 Antibody [Unconjugated]
(3 Publications)
Species: Hu, Mu, Rt
Applications: IHC, KO, WB
SGSM3 Antibody
NBP2-13304
Immunohistochemistry-Paraffin SGSM3 Antibody
Immunocytochemistry/ Immunofluorescence SGSM3 Antibody
Western Blot SGSM3 Antibody
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
PRAK Antibody
NBP1-87231
Immunohistochemistry-Paraffin PRAK Antibody
Immunohistochemistry-Paraffin PRAK Antibody
Immunohistochemistry-Paraffin PRAK Antibody
(1 Publication)
Species: Hu
Applications: ICC/IF, IHC, IHC-P
ERK4/MAPK4
Antibody (OTI2D7)
NBP2-45743
Immunofluorescence ERK4/MAPK4 Antibody (OTI2D7)
Immunohistochemistry ERK4/MAPK4 Antibody (OTI2D7)
Immunohistochemistry ERK4/MAPK4 Antibody (OTI2D7)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
p38 gamma/SAPK3
Antibody [Unconjugate
AF1347
Immunocytochemistry p38 gamma/SAPK3 Antibody [Unconjugated]
Simple Western p38 gamma/SAPK3 Antibody [Unconjugated]
Western Blot p38 gamma/SAPK3 Antibody [Unconjugated]
(8 Publications)
Species: Hu, Mu, Rt
Applications: ICC, IHC, Simple Western, WB
ERK5/BMK1 Antibody
[Unconjugated]
AF2848
Western Blot ERK5/BMK1 Antibody [Unconjugated]
Immunocytochemistry ERK5/BMK1 Antibody [Unconjugated]
(4 Publications)
Species: Hu, Mu
Applications: ICC, WB
EphB2 Antibody
[Unconjugated]
AF467
Simple Western EphB2 Antibody [Unconjugated]
Western Blot EphB2 Antibody [Unconjugated]
Immunocytochemistry EphB2 Antibody [Unconjugated]
(5 Reviews)
(62 Publications)
Species: Hu, Mu
Applications: CyTOF-ready, Flow, ICC, IHC, Simple Western, WB
p38 alpha Antibody
[Unconjugated]
AF8691
Simple Western p38 alpha Antibody [Unconjugated]
Western Blot p38 alpha Antibody [Unconjugated]
Immunohistochemistry p38 alpha Antibody [Unconjugated]
(1 Review)
(11 Publications)
Species: Hu, Mu, Rt
Applications: IHC, KO, Simple Western, WB
Crk Antibody
(3G11E8) - BSA Free
NBP2-37568
ELISA Crk Antibody (3G11E8) - BSA Free
Western Blot Crk Antibody (3G11E8) - BSA Free
Western Blot Crk Antibody (3G11E8) - BSA Free
Species: Hu
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
GRAP2 Antibody
(OTI1G2)
NBP2-00764
Flow Cytometry GRAP2 Antibody (OTI1G2)
Flow Cytometry GRAP2 Antibody (OTI1G2)
Western Blot GRAP2 Antibody (OTI1G2)
Species: Hu, Mu, Rt
Applications: Flow, IHC, IHC-P, WB
AIMP2 Antibody
NBP1-81575
Immunohistochemistry-Paraffin AIMP2 Antibody
Immunohistochemistry-Paraffin AIMP2 Antibody
Immunohistochemistry-Paraffin AIMP2 Antibody
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
POLDIP2 Antibody
NBP1-68874
Western Blot POLDIP2 Antibody
Western Blot POLDIP2 Antibody
(1 Publication)
Species: Hu, Mu, Rt
Applications: PEP-ELISA, WB
AHSA1 Antibody
(1A2-A8)
H00010598-M01
Western Blot AHSA1 Antibody (1A2-A8)
Sandwich ELISA AHSA1 Antibody (1A2-A8)
(6 Publications)
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, S-ELISA, WB
p21/CIP1/CDKN1A
Antibody (WA-1)
NBP2-29463
Immunohistochemistry-Paraffin p21/CIP1/CDKN1A Antibody (WA-1)
(24 Publications)
Species: Pm, Hu, Pm, Mu
Applications: Flow, ICC/IF, IHC, IHC-P, WB
JNK1 Antibody
(228601) [Unconjugate
MAB17761
Western Blot JNK1 Antibody (228601) [Unconjugated]
Western Blot JNK1 Antibody (228601) [Unconjugated]
Immunocytochemistry JNK1 Antibody (228601) [Unconjugated]
(1 Review)
(3 Publications)
Species: Hu, Mu, Rt
Applications: ICC, WB
Raf-1 Antibody
(563002) [Unconjugate
MAB4540
Western Blot Raf-1 Antibody (563002) [Unconjugated]
Immunohistochemistry Raf-1 Antibody (563002) [Unconjugated]
Simple Western Raf-1 Antibody (563002) [Unconjugated]
(2 Publications)
Species: Hu, Mu, Rt
Applications: IHC, WB
ERK3/MAPK6
Antibody (OTI5E1)
NBP1-47854
ERK3/MAPK6 Antibody (OTI5E1)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC

Publications for ERK3/MAPK6 Antibody (NBP1-47854) (0)

There are no publications for ERK3/MAPK6 Antibody (NBP1-47854).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for ERK3/MAPK6 Antibody (NBP1-47854) (0)

There are no reviews for ERK3/MAPK6 Antibody (NBP1-47854). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ERK3/MAPK6 Antibody (NBP1-47854) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional ERK3/MAPK6 Products

Research Areas for ERK3/MAPK6 Antibody (NBP1-47854)

Find related products by research area.

Blogs on ERK3/MAPK6

There are no specific blogs for ERK3/MAPK6, but you can read our latest blog posts.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our ERK3/MAPK6 Antibody (OTI5E1) and receive a gift card or discount.


Bioinformatics

Gene Symbol MAPK6
Entrez