![Western Blot: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1375) - Azide and BSA Free [NBP2-54452] - Y79, HeLa and HepG2 Cell Lysate using NSE, gamma Monoclonal Antibody (ENO2/1375).](http://images.novusbio.com/fullsize/Enolase-2-Neuron-specific-Enolase-Antibody-ENO2-1375-Azide-and-BSA-Free-Western-Blot-NBP2-54452-img0005.jpg "Western Blot: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1375) - Azide and BSA Free [NBP2-54452] - Y79, HeLa and HepG2 Cell Lysate using NSE, gamma Monoclonal Antibody (ENO2/1375).")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, IHC, CyTOF-ready |
| Clone | ENO2/1375 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-47635). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | A synthetic peptide of human Enolase 2/Neuron-specific Enolase (around aa416-433) (exact sequence is proprietary) (Uniprot: P09104) |
| Localization | Cytoplasmic |
| Marker | Neuroendocrine Marker |
| Isotype | IgG2b Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ENO2 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 0.2-0.4ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Theoretical MW | 50 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Enolase 2/Neuron-specific Enolase Antibody (NBP2-54452)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | ENO2 |
![Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1375) - Azide and BSA Free [NBP2-54452] - Formalin-fixed, paraffin-embedded Rat Heart stained with Formalin-fixed, paraffin-embedded Rat Heart stained with NSE gamma Monoclonal Antibody (ENO2/1375).](http://images.novusbio.com/fullsize/Enolase-2-Neuron-specific-Enolase-Antibody-ENO2-1375-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54452-img0004.jpg "Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1375) - Azide and BSA Free [NBP2-54452] - Formalin-fixed, paraffin-embedded Rat Heart stained with Formalin-fixed, paraffin-embedded Rat Heart stained with NSE gamma Monoclonal Antibody (ENO2/1375).")
![Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1375) - Azide and BSA Free [NBP2-54452] - Formalin-fixed, paraffin-embedded Human Cerebellum stained with NSE gamma Monoclonal Antibody (ENO2/1375).](http://images.novusbio.com/fullsize/Enolase-2-Neuron-specific-Enolase-Antibody-ENO2-1375-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54452-img0001.jpg "Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1375) - Azide and BSA Free [NBP2-54452] - Formalin-fixed, paraffin-embedded Human Cerebellum stained with NSE gamma Monoclonal Antibody (ENO2/1375).")
![Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1375) - Azide and BSA Free [NBP2-54452] - Formalin-fixed, paraffin-embedded Human Pheochromocytoma stained with NSE gamma Monoclonal Antibody (ENO2/1375).](http://images.novusbio.com/fullsize/Enolase-2-Neuron-specific-Enolase-Antibody-ENO2-1375-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54452-img0002.jpg "Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1375) - Azide and BSA Free [NBP2-54452] - Formalin-fixed, paraffin-embedded Human Pheochromocytoma stained with NSE gamma Monoclonal Antibody (ENO2/1375).")
![Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1375) - Azide and BSA Free [NBP2-54452] - Formalin-fixed, paraffin-embedded Mouse Pancreas stained with NSE gamma Monoclonal Antibody (ENO2/1375).](http://images.novusbio.com/fullsize/Enolase-2-Neuron-specific-Enolase-Antibody-ENO2-1375-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54452-img0003.jpg "Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1375) - Azide and BSA Free [NBP2-54452] - Formalin-fixed, paraffin-embedded Mouse Pancreas stained with NSE gamma Monoclonal Antibody (ENO2/1375).")
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