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Rat Endoglin/CD105 ELISA Kit (Colorimetric)

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ELISA: Rat Endoglin/CD105 ELISA Kit (Colorimetric) [NBP2-82135] - Samples were spiked with high concentrations of Rat Endoglin/CD105 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
ELISA: Rat Endoglin/CD105 ELISA Kit (Colorimetric) [NBP2-82135] - Samples were spiked with high concentrations of Rat Endoglin/CD105 and diluted with Reference Standard & Sample Diluent to produce samples with values ...read more
ELISA: Rat Endoglin/CD105 ELISA Kit (Colorimetric) [NBP2-82135] - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, Plasma, Cell supernatant
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.09 ng/mL

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Rat Endoglin/CD105 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Rat Endoglin/CD105 in samples. No significant cross-reactivity or interference between Rat Endoglin/CD105 and analogues was observed.
Standard Curve Range
0.16 - 10 ng/mL
Sensitivity
0.09 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 5%
Intra-Assay
CV% < 6%
Spike Recovery
average = 99% for serum; average = 102% for EDTA plasma; average = 92% for cell culture media
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
ENG

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Micro ELISA Plate, dismountable (8 wells x 12 strips); Reference Standard (2 vials); Concentrated (100X) Biotinylated Detection Ab (120 uL); Concentrated (100X) HRP Conjugate (120 uL); Reference Standard & Sample Diluent (20 mL); Biotinylated Detection Ab

Alternate Names for Rat Endoglin/CD105 ELISA Kit (Colorimetric)

  • CD105 antigen
  • CD105
  • Endoglin
  • ENDOsler-Rendu-Weber syndrome 1
  • ENG
  • HHT1FLJ41744
  • ORW
  • ORW1

Background

CD105 is a 90 kD homodimeric type I integral membrane glycoprotein, also known as endoglin. It is expressed on endothelial cells (especially on angiogenic endothelial cells) and up-regulated by hypoxia, activated monocytes, macrophages, bone marrow stromal cells, and some cytotrophoblasts. CD105 is a receptor for TGF- szlig1, TGF- szlig3 and modulates TGF- szlig signaling by interacting with TGF- szlig receptors I and/or II. CD105 also binds other growth factors such as actvin A, BMP-2 and -7. CD105 has been show to be a useful marker for identifying proliferating endothelium involved in tumor angiogenesis and can be used for tumor imaging, prognosis, as well as has therapeutic potential for some solid tumors and other angiogenic diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Rat Endoglin/CD105
ELISA Kit (Colorimetr ...
NBP2-82135
Rat Endoglin/CD105 ELISA Kit (Colorimetric)
Species: Rt
Applications: ELISA

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Product General Protocols

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FAQs for Endoglin/CD105 ELISA Kit (NBP2-82135). (Showing 1 - 2 of 2 FAQ).

  1. I wonder if you have a CD105 or CD34 antibody suitable for IHC that is specific for human and do not bind mouse?
    • We do not have any anti-human CD34 or CD105 antibodies that are confirmed to NOT detect the mouse protein. When we have tested an antibody and confirmed that it will not react with mouse samples, we will add Mu(-) to the datasheet, and unfortunately all of our CD105 and CD34 antibodies will either detect the mouse protein, or they have not been used in mouse samples before.
  2. We are interested in CD105 antibodies that could react with dog, please let me know which products in your catalog you would recommend.
    • We do not currently carry any CD105 antibodies that have been validated for use in dog samples. However, if you are interested in testing any of our CD105 antibodies with dog samples you would be eligible for our Innovators Reward Program, in which you can get a discount voucher for the purchase price of the product. Please contact us at innovators@novusbio.com with any questions regarding this program.

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Bioinformatics

Gene Symbol ENG