![Flow Cytometry: Endoglin/CD105 Antibody (SN6) [NB100-65601] - A surface stain was performed on HeLa cells with Endoglin/CD105 Antibody (SN6) NB100-65601PE and a matched isotype control. Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-SN6-Flow-Cytometry-NB100-65601-img0008.jpg "Flow Cytometry: Endoglin/CD105 Antibody (SN6) [NB100-65601] - A surface stain was performed on HeLa cells with Endoglin/CD105 Antibody (SN6) NB100-65601PE and a matched isotype control. Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, Flow, IHC, IP |
| Clone | SN6 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Clone SN6 was used by HLDA to establish CD designation. |
| Immunogen | Partially purified cell membrane antigens from fresh leukemia cells |
| Marker | Neo-endothelial Cells Marker |
| Specificity | Recognizes the human CD105 cell surface antigen, a glycoprotein homodimer of 95kD subunits. CD105 is also known as endoglin, and is expressed by endothelial cells, activated monocytes and some leukemia cells. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ENG |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. The use of acetone fixation for frozen sections is recommended. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
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| Publication using NB100-65601 | Applications | Species |
|---|---|---|
| Cavalcanti BN. Pulp biology: aggression repair. Thesis. 2014-01-01 (WB, Human) Details: Endoglin/CD105 antibody used for WB on lysates of unselected pulp cell populations (uDPC) and selected dental pulp stem cells (sDPC) isolated from a single extracted third molar of human patient (Figure 2B) |
WB | Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for Endoglin/CD105 Antibody (NB100-65601)Find related products by research area.
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![Flow Cytometry: Endoglin/CD105 Antibody (SN6) [NB100-65601] - Staining of human peripheral blood monocytes with Mouse anti Human CD105:Alexa Fluor 488](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-SN6-Flow-Cytometry-NB100-65601-img0002.jpg "Flow Cytometry: Endoglin/CD105 Antibody (SN6) [NB100-65601] - Staining of human peripheral blood monocytes with Mouse anti Human CD105:Alexa Fluor 488")
![Flow Cytometry: Endoglin/CD105 Antibody (SN6) [NB100-65601] - Staining of human peripheral blood monocytes with Mouse anti Human CD105:Alexa Fluor 647](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-SN6-Flow-Cytometry-NB100-65601-img0003.jpg "Flow Cytometry: Endoglin/CD105 Antibody (SN6) [NB100-65601] - Staining of human peripheral blood monocytes with Mouse anti Human CD105:Alexa Fluor 647")
![Flow Cytometry: Endoglin/CD105 Antibody (SN6) [NB100-65601] - Staining of KG1 cells with Mouse anti Human CD105RPE](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-SN6-Flow-Cytometry-NB100-65601-img0004.jpg "Flow Cytometry: Endoglin/CD105 Antibody (SN6) [NB100-65601] - Staining of KG1 cells with Mouse anti Human CD105RPE")
![Flow Cytometry: Endoglin/CD105 Antibody (SN6) [NB100-65601] - Staining of KG1 cells with Mouse anti Human CD105](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-SN6-Flow-Cytometry-NB100-65601-img0001.jpg "Flow Cytometry: Endoglin/CD105 Antibody (SN6) [NB100-65601] - Staining of KG1 cells with Mouse anti Human CD105")
![Flow Cytometry: Endoglin/CD105 Antibody (SN6) [NB100-65601] - Analysis using the FITC conjugate of NB100-65601. Staining of KG1 cells.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-SN6-Flow-Cytometry-NB100-65601-img0006.jpg "Flow Cytometry: Endoglin/CD105 Antibody (SN6) [NB100-65601] - Analysis using the FITC conjugate of NB100-65601. Staining of KG1 cells.")
![Flow Cytometry: Endoglin/CD105 Antibody (SN6) [NB100-65601] - A cell surface stain was performed on HeLa cells with Endoglin/CD105 antibody (SN6) NB100-65601PCP (blue) and a matched isotype control NBP2-27287AF647 (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Peridinin-Chlorophyll-Protein complex.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-SN6-Flow-Cytometry-NB100-65601-img0007.jpg "Flow Cytometry: Endoglin/CD105 Antibody (SN6) [NB100-65601] - A cell surface stain was performed on HeLa cells with Endoglin/CD105 antibody (SN6) NB100-65601PCP (blue) and a matched isotype control NBP2-27287AF647 (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Peridinin-Chlorophyll-Protein complex.")
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