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Endoglin/CD105 Antibody (3A9) [Janelia Fluor® 549]

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clone
3A9
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 549

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-22122AF350
Alexa Fluor 405
NBP2-22122AF405
Alexa Fluor 488
NBP2-22122AF488
Alexa Fluor 532
NBP2-22122AF532
Alexa Fluor 594
NBP2-22122AF594
Alexa Fluor 647
NBP2-22122AF647
Alexa Fluor 700
NBP2-22122AF700
Alexa Fluor 750
NBP2-22122AF750
Biotin
NBP2-22122B
CoraFluor 1
NBP2-22122CL1
DyLight 350
NBP2-22122UV
DyLight 405
NBP2-22122V
DyLight 488
NBP2-22122G
DyLight 550
NBP2-22122R
DyLight 594
NBP2-22122DL594
DyLight 650
NBP2-22122C
DyLight 680
NBP2-22122FR
DyLight 755
NBP2-22122IR
FITC
NBP2-22122F
HRP
NBP2-22122H
Janelia Fluor 646
NBP2-22122JF646
Unconjugated
NBP2-22122
mFluor Violet 450 SE
NBP2-22122MFV450
mFluor Violet 500 SE
NBP2-22122MFV500
mFluor Violet 610 SE
NBP2-22122MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-22122
BSA Free
NBP2-22122
BSA Free
NBP2-22122SS
Azide and BSA Free
NBP2-80719
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Endoglin/CD105 Antibody (3A9) [Janelia Fluor® 549] Summary

Immunogen
Purified recombinant fragment of human CD105 expressed in E. coli. [Uniprot: P17813]
Marker
Neo-endothelial Cells Marker
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ENG
Purity
Ammonium sulfate precipitation
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 27322553).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Ammonium sulfate precipitation

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Endoglin/CD105 Antibody (3A9) [Janelia Fluor® 549]

  • CD105 antigen
  • CD105
  • Endoglin
  • ENDOsler-Rendu-Weber syndrome 1
  • ENG
  • HHT1FLJ41744
  • ORW
  • ORW1

Background

CD105 is a 90 kD homodimeric type I integral membrane glycoprotein, also known as endoglin. It is expressed on endothelial cells (especially on angiogenic endothelial cells) and up-regulated by hypoxia, activated monocytes, macrophages, bone marrow stromal cells, and some cytotrophoblasts. CD105 is a receptor for TGF- szlig1, TGF- szlig3 and modulates TGF- szlig signaling by interacting with TGF- szlig receptors I and/or II. CD105 also binds other growth factors such as actvin A, BMP-2 and -7. CD105 has been show to be a useful marker for identifying proliferating endothelium involved in tumor angiogenesis and can be used for tumor imaging, prognosis, as well as has therapeutic potential for some solid tumors and other angiogenic diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Endoglin/CD105
Antibody (3A9) [Janel ...
NBP2-22122JF549
Endoglin/CD105 Antibody (3A9) [Janelia Fluor® 549]
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Endoglin/CD105 Antibody (NBP2-22122JF549). (Showing 1 - 2 of 2 FAQ).

  1. I wonder if you have a CD105 or CD34 antibody suitable for IHC that is specific for human and do not bind mouse?
    • We do not have any anti-human CD34 or CD105 antibodies that are confirmed to NOT detect the mouse protein. When we have tested an antibody and confirmed that it will not react with mouse samples, we will add Mu(-) to the datasheet, and unfortunately all of our CD105 and CD34 antibodies will either detect the mouse protein, or they have not been used in mouse samples before.
  2. We are interested in CD105 antibodies that could react with dog, please let me know which products in your catalog you would recommend.
    • We do not currently carry any CD105 antibodies that have been validated for use in dog samples. However, if you are interested in testing any of our CD105 antibodies with dog samples you would be eligible for our Innovators Reward Program, in which you can get a discount voucher for the purchase price of the product. Please contact us at innovators@novusbio.com with any questions regarding this program.

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Bioinformatics

Gene Symbol ENG