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eIF4A1 Antibody (SR1793)

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Western Blot: eIF4A1 Antibody (SR1793) [NBP3-21969] - Western blot analysis using NBP3-21969 in MCF7 cell lysate.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF
Clone
SR1793
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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eIF4A1 Antibody (SR1793) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
A synthesized peptide derived from human eIF4A1 (Uniprot #: P60842)
Specificity
Detects endogenous levels of total eIF4A1
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
EIF4A1
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:50
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Western Blot 1:500-1:2000
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.4, 150mM NaCl, 50% glycerol.
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for eIF4A1 Antibody (SR1793)

  • ATP-dependent RNA helicase eIF4A-1
  • DDX2Aeukaryotic initiation factor 4A-I
  • EC 3.6.1
  • EC 3.6.4.13
  • EIF4A
  • EIF-4A
  • eIF-4A-I
  • eIF4A-I
  • eukaryotic initiation factor 4AI
  • eukaryotic translation initiation factor 4A
  • eukaryotic translation initiation factor 4A, isoform 1
  • eukaryotic translation initiation factor 4A1

Background

ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and isrequired for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondarystructures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, andsubsequent scanning for the initiator codon

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for eIF4A1 Antibody (NBP3-21969) (0)

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Secondary Antibodies

 

Isotype Controls

Additional eIF4A1 Products

Array NBP3-21969

Research Areas for eIF4A1 Antibody (NBP3-21969)

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Bioinformatics

Gene Symbol EIF4A1