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eIF2B1 Antibody

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Western Blot: eIF2B1 Antibody [NBP2-16292] - Sample (30 ug of whole cell lysate) A: A431 B: Jurkat 12% SDS PAGE gel, diluted at 1:10000.
Western Blot: eIF2B1 Antibody [NBP2-16292] - Sample (30 ug of whole cell lysate) A: A431 B: Jurkat 12% SDS PAGE gel, diluted at 1:10000.
Immunocytochemistry/ Immunofluorescence: eIF2B1 Antibody [NBP2-16292] - A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EIF2B1 protein stained by EIF2B1 antibody diluted at 1:500. Blue: Hoechst ...read more
Immunohistochemistry-Paraffin: eIF2B1 Antibody [NBP2-16292] - Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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eIF2B1 Antibody Summary

Immunogen
Full length human eIF2B1 Recombinant protein.
Predicted Species
Mouse (94%), Rat (92%), Bovine (95%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EIF2B1
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:5000-1:20000
Theoretical MW
34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for eIF2B1 Antibody

  • eIF-2B GDP-GTP exchange factor subunit alpha
  • EIF2B
  • EIF-2B
  • EIF-2Balpha
  • EIF2BAMGC117409
  • eukaryotic translation initiation factor 2B, subunit 1 (alpha, 26kD)
  • eukaryotic translation initiation factor 2B, subunit 1 alpha, 26kDa
  • MGC125868
  • MGC125869
  • translation initiation factor eIF-2B subunit alpha

Background

This gene encodes one of five subunits of eukaryotic translation initiation factor 2B (EIF2B), a GTP exchange factor for eukaryotic initiation factor 2 and an essential regulator for protein synthesis. Mutations in this gene and the genes encoding other EIF2B subunits have been associated with leukoencephalopathy with vanishing white matter. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol EIF2B1
Uniprot