![Western Blot: eIF2 alpha/EIF2S1 Antibody (PSH04-29) [NBP3-32296] - Western blot analysis of eIF2 alpha/EIF2S1 on different lysates with Rabbit anti-eIF2 alpha/EIF2S1 antibody (NBP3-32296) at 1/2,000 dilution. Lane 1: MCF7 cell lysate Lane 2: HepG2 cell lysate Lane 3: HeLa cell lysate Lane 4: COS-1 cell lysate Lane 5: A549 cell lysate Lane 6: RAW264.7 cell lysate Lane 7: C6 cell lysate Lane 8: Mouse kidney tissue lysate Lane 9: Mouse spleen tissue lysate Lane 10: Rat kidney tissue lysate Lane 11: Rat spleen tissue lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 36 kDa Observed band size: 36 kDa Exposure time: 59 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32296) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32296_rabbit-eif2-alpha-eif2s1-mab-psh04-29-27820241628526.jpg "Western Blot: eIF2 alpha/EIF2S1 Antibody (PSH04-29) [NBP3-32296] - Western blot analysis of eIF2 alpha/EIF2S1 on different lysates with Rabbit anti-eIF2 alpha/EIF2S1 antibody (NBP3-32296) at 1/2,000 dilution. Lane 1: MCF7 cell lysate Lane 2: HepG2 cell lysate Lane 3: HeLa cell lysate Lane 4: COS-1 cell lysate Lane 5: A549 cell lysate Lane 6: RAW264.7 cell lysate Lane 7: C6 cell lysate Lane 8: Mouse kidney tissue lysate Lane 9: Mouse spleen tissue lysate Lane 10: Rat kidney tissue lysate Lane 11: Rat spleen tissue lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 36 kDa Observed band size: 36 kDa Exposure time: 59 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32296) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
| Reactivity | Hu, Mu, Rt, PmSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC |
| Clone | PSH04-29 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant protein within human eIF2 alpha/eIF2 alpha/EIF2S1 aa 1-315 / 315. (Uniprot: P05198) |
| Localization | Cytoplasm, Stress granule. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | EIF2S1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 36 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH7.4), 0.1% BSA and 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for eIF2 alpha/EIF2S1 Antibody (NBP3-32296)Find related products by research area.
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| Gene Symbol | EIF2S1 |
![Immunohistochemistry: eIF2 alpha/EIF2S1 Antibody (PSH04-29) [NBP3-32296] - Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-eIF2 alpha/EIF2S1 antibody (NBP3-32296) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32296) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32296_rabbit-eif2-alpha-eif2s1-mab-psh04-29-29820241420926.jpg "Immunohistochemistry: eIF2 alpha/EIF2S1 Antibody (PSH04-29) [NBP3-32296] - Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-eIF2 alpha/EIF2S1 antibody (NBP3-32296) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32296) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: eIF2 alpha/EIF2S1 Antibody (PSH04-29) [NBP3-32296] - Immunocytochemistry analysis of HepG2 cells labeling eIF2 alpha/EIF2S1 with Rabbit anti-eIF2 alpha/EIF2S1 antibody (NBP3-32296) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-eIF2 alpha/EIF2S1 antibody (NBP3-32296) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32296_rabbit-eif2-alpha-eif2s1-mab-psh04-29-29202410484850.jpg "Immunocytochemistry/ Immunofluorescence: eIF2 alpha/EIF2S1 Antibody (PSH04-29) [NBP3-32296] - Immunocytochemistry analysis of HepG2 cells labeling eIF2 alpha/EIF2S1 with Rabbit anti-eIF2 alpha/EIF2S1 antibody (NBP3-32296) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-eIF2 alpha/EIF2S1 antibody (NBP3-32296) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.")
![Flow Cytometry: eIF2 alpha/EIF2S1 Antibody (PSH04-29) [NBP3-32296] - Flow cytometric analysis of HepG2 cells labeling eIF2 alpha/EIF2S1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32296, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32296_rabbit-eif2-alpha-eif2s1-mab-psh04-29-2920241645323.jpg "Flow Cytometry: eIF2 alpha/EIF2S1 Antibody (PSH04-29) [NBP3-32296] - Flow cytometric analysis of HepG2 cells labeling eIF2 alpha/EIF2S1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32296, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")