Home » eIF2B3 » eIF2B3 Antibodies » eIF2B3 Antibody (OTI1A4)
Novus Biologicals products are now on bio-techne.com

eIF2B3 Antibody (OTI1A4)

 Submit a Review

Images

 
(
Enlarge)
Western Blot: eIF2B3 Antibody (1A4) [NBP2-01370] Analysis of extracts (35ug) from 9 different cell lines by using anti-eIF2B3 monoclonal antibody.
Western Blot: eIF2B3 Antibody (1A4) [NBP2-01370] Analysis of extracts (35ug) from 9 different cell lines by using anti-eIF2B3 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: eIF2B3 Antibody (1A4) [NBP2-01370] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY eIF2B3.
Immunohistochemistry-Paraffin: eIF2B3 Antibody (1A4) [NBP2-01370] - Staining of paraffin-embedded Human prostate tissue using anti-eIF2B3 mouse monoclonal antibody.
Western Blot: eIF2B3 Antibody (1A4) [NBP2-01370] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY eIF2B3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunohistochemistry-Paraffin: eIF2B3 Antibody (1A4) [NBP2-01370] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-eIF2B3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: eIF2B3 Antibody (1A4) [NBP2-01370] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-eIF2B3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: eIF2B3 Antibody (1A4) [NBP2-01370] - Staining of paraffin-embedded Human endometrium tissue using anti-eIF2B3 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: eIF2B3 Antibody (1A4) [NBP2-01370] - Staining of paraffin-embedded Human pancreas tissue using anti-eIF2B3 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, Rt, CaSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
OTI1A4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.7 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-71397AF350
Alexa Fluor 405
NBP2-71397AF405
Alexa Fluor 488
NBP2-71397AF488
Alexa Fluor 532
NBP2-71397AF532
Alexa Fluor 594
NBP2-71397AF594
Alexa Fluor 647
NBP2-71397AF647
Alexa Fluor 700
NBP2-71397AF700
Alexa Fluor 750
NBP2-71397AF750
Allophycocyanin
NBP2-71397APC
Biotin
NBP2-71397B
CoraFluor 1
NBP2-71397CL1
DyLight 350
NBP2-71397UV
DyLight 405
NBP2-71397V
DyLight 488
NBP2-71397G
DyLight 550
NBP2-71397R
DyLight 594
NBP2-71397DL594
DyLight 650
NBP2-71397C
DyLight 680
NBP2-71397FR
DyLight 755
NBP2-71397IR
FITC
NBP2-71397F
HRP
NBP2-71397H
Janelia Fluor 525
NBP2-71397JF525
Janelia Fluor 549
NBP2-71397JF549
Janelia Fluor 646
NBP2-71397JF646
Janelia Fluor 669
NBP2-71397JF669
PE
NBP2-71397PE
PerCP
NBP2-71397PCP
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-71397
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

eIF2B3 Antibody (OTI1A4) Summary

Immunogen
Full length human recombinant protein of human EIF2B3(NP_065098) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
EIF2B3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
Theoretical MW
50.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.7 mg/ml
Purity
Immunogen affinity purified

Alternate Names for eIF2B3 Antibody (OTI1A4)

  • eIF-2B GDP-GTP exchange factor subunit gamma
  • EIF-2B
  • EIF2Bgamma
  • eukaryotic translation initiation factor 2B, subunit 3 (gamma, 58kD)
  • eukaryotic translation initiation factor 2B, subunit 3 gamma, 58kDa
  • translation initiation factor eIF-2B subunit gamma

Background

The protein encoded by this gene is one of the subunits of initiation factor eIF2B, which catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP. It has also been found to function as a cofactor of hepatitis C virus internal ribosome entry site-mediated translation. Mutations in this gene have been associated with leukodystrophy with vanishing white matter. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

eIF2 alpha/EIF2S1
Antibody (OTI3H4)
NBP2-02669
Flow Cytometry eIF2 alpha/EIF2S1 Antibody (OTI3H4)
Western Blot eIF2 alpha/EIF2S1 Antibody (OTI3H4)
Immunohistochemistry-Paraffin eIF2 alpha/EIF2S1 Antibody (OTI3H4)
Species: Ca, Hu, Pm, Mu, Pm, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
SOS2 Antibody
[Unconjugated]
AF6260
Western Blot SOS2 Antibody [Unconjugated]
(1 Publication)
Species: Hu
Applications: WB
SOS1 Antibody
(SOS-01) - BSA Free
NB110-68800
Western Blot SOS1 Antibody (SOS-01) - BSA Free
Immunocytochemistry/ Immunofluorescence SOS1 Antibody (SOS-01) - BSA Free
Immunocytochemistry/ Immunofluorescence SOS1 Antibody (SOS-01) - BSA Free
Species: Hu, Mu
Applications: ICC/IF, WB
RASGRF1 Antibody
NBP3-13291
Western Blot RASGRF1 Antibody
Immunohistochemistry-Paraffin RASGRF1 Antibody
Immunohistochemistry-Paraffin RASGRF1 Antibody
Species: Mu, Rt
Applications: WB
EIF2AK1 Antibody
NBP1-83210
Immunohistochemistry-Paraffin EIF2AK1 Antibody
Immunocytochemistry/ Immunofluorescence EIF2AK1 Antibody
Western Blot EIF2AK1 Antibody
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
eIF2B1 Antibody
NBP2-16292
Immunocytochemistry/ Immunofluorescence eIF2B1 Antibody
Western Blot eIF2B1 Antibody
Immunohistochemistry-Paraffin eIF2B1 Antibody
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
Insulin Antibody
(182410) [Unconjugate ...
MAB1417
Immunocytochemistry Insulin Antibody (182410) [Unconjugated]
Immunohistochemistry Insulin Antibody (182410) [Unconjugated]
(24 Publications)
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
EIF2B2 Antibody
NBP3-38367
Immunocytochemistry/ Immunofluorescence EIF2B2 Antibody
Immunohistochemistry EIF2B2 Antibody
Immunohistochemistry EIF2B2 Antibody
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, IP, WB
eIF2B4 Antibody
NBP3-24813
Western Blot eIF2B4 Antibody
Immunocytochemistry/ Immunofluorescence eIF2B4 Antibody
Immunohistochemistry-Paraffin eIF2B4 Antibody
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
GCN2 [p Thr899]
Antibody
AF7605
Western Blot GCN2 [p Thr899] Antibody
(2 Publications)
Species: Hu
Applications: WB
EIF2 beta Antibody
(2F3)
H00008894-M09
Immunocytochemistry/ Immunofluorescence EIF2 beta Antibody (2F3)
Immunohistochemistry-Paraffin EIF2 beta Antibody (2F3)
Western Blot EIF2 beta Antibody (2F3)
(1 Publication)
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
GEF-H1 Antibody
NBP2-21577
Western Blot GEF-H1 Antibody
Western Blot GEF-H1 Antibody
Western Blot GEF-H1 Antibody
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD, WB
SLC2A4RG Antibody
(4C10)
H00056731-M02
Western Blot SLC2A4RG Antibody (4C10)
Immunocytochemistry/ Immunofluorescence SLC2A4RG Antibody (4C10)
Species: Hu
Applications: ELISA, ICC/IF, WB
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(817 Publications)
Species: Hu
Applications: BA
eIF2B epsilon
Antibody (5M2D4)
NBP3-16674
Western Blot eIF2B epsilon Antibody (5M2D4)
Immunocytochemistry/ Immunofluorescence eIF2B epsilon Antibody (5M2D4)
Immunohistochemistry-Paraffin eIF2B epsilon Antibody (5M2D4)
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
4EBP1 Antibody
NB200-157
Knockout Validated 4EBP1 Antibody
Western Blot 4EBP1 Antibody
Western Blot 4EBP1 Antibody
(45 Publications)
Species: Hu
Applications: IHC, IHC-P, KO, WB
eEF-2 Antibody
(4E2Z5)
NBP3-16866
Immunohistochemistry-Paraffin eEF-2 Antibody (4E2Z5)
Immunocytochemistry/ Immunofluorescence eEF-2 Antibody (4E2Z5)
Immunocytochemistry/ Immunofluorescence eEF-2 Antibody (4E2Z5)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
eIF2B3 Antibody
(OTI1A4)
NBP2-01370
eIF2B3 Antibody (OTI1A4)
Species: Hu, Mu, Rt, Ca
Applications: WB, ICC/IF, IHC

Publications for eIF2B3 Antibody (NBP2-01370) (0)

There are no publications for eIF2B3 Antibody (NBP2-01370).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for eIF2B3 Antibody (NBP2-01370) (0)

There are no reviews for eIF2B3 Antibody (NBP2-01370). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for eIF2B3 Antibody (NBP2-01370) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional eIF2B3 Products

Blogs on eIF2B3

There are no specific blogs for eIF2B3, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Western blot shows detection of Mouse Anti-Human/Mouse/Rat STAT3 Monoclonal Antibody (MAB1799) using a 1:1000 dilution of HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Goat anti-Mouse IgG Secondary Antibody [HRP]
HAF007
SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.
Mouse IgG1 Isotype Control (MG1)
NBP1-97005-0.5mg

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our eIF2B3 Antibody (OTI1A4) and receive a gift card or discount.


Bioinformatics

Gene Symbol EIF2B3
Entrez