| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70626 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3997-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB39971-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB3997-SP | |||
![Western Blot: EIF2S1 Antibody (3H4) [NBP2-02669] Analysis of extracts (35ug) from 9 different cell lines by using anti-eIF2A monoclonal antibody.](http://images.novusbio.com/fullsize/EIF2S1-Antibody-3H4-Western-Blot-NBP2-02669-img0009.jpg "Western Blot: EIF2S1 Antibody (3H4) [NBP2-02669] Analysis of extracts (35ug) from 9 different cell lines by using anti-eIF2A monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: EIF2S1 Antibody (3H4) [NBP2-02669] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY eIF2A.](http://images.novusbio.com/fullsize/EIF2S1-Antibody-3H4-Immunocytochemistry-Immunofluorescence-NBP2-02669-img0008.jpg)
![Immunohistochemistry-Paraffin: EIF2S1 Antibody (3H4) [NBP2-02669] - Staining of paraffin-embedded Human prostate tissue using anti-eIF2A mouse monoclonal antibody.](http://images.novusbio.com/fullsize/EIF2S1-Antibody-3H4-Immunohistochemistry-Paraffin-NBP2-02669-img0007.jpg)
![Flow Cytometry: EIF2S1 Antibody (3H4) [NBP2-02669] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-eIF2A antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/EIF2S1-Antibody-3H4-Flow-Cytometry-NBP2-02669-img0001.jpg)
![Western Blot: EIF2S1 Antibody (3H4) [NBP2-02669] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY eIF2A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-eIF2A.](http://images.novusbio.com/fullsize/EIF2S1-Antibody-3H4-Western-Blot-NBP2-02669-img0002.jpg)
![Immunohistochemistry-Paraffin: EIF2S1 Antibody (3H4) [NBP2-02669] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-eIF2A mouse monoclonal antibody.](http://images.novusbio.com/fullsize/EIF2S1-Antibody-3H4-Immunohistochemistry-Paraffin-NBP2-02669-img0003.jpg)
![Immunohistochemistry-Paraffin: EIF2S1 Antibody (3H4) [NBP2-02669] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-eIF2A mouse monoclonal antibody.](http://images.novusbio.com/fullsize/EIF2S1-Antibody-3H4-Immunohistochemistry-Paraffin-NBP2-02669-img0004.jpg)
![Immunohistochemistry-Paraffin: EIF2S1 Antibody (3H4) [NBP2-02669] - Staining of paraffin-embedded Human Kidney tissue using anti-eIF2A mouse monoclonal antibody.](http://images.novusbio.com/fullsize/EIF2S1-Antibody-3H4-Immunohistochemistry-Paraffin-NBP2-02669-img0005.jpg)
![Immunohistochemistry-Paraffin: EIF2S1 Antibody (3H4) [NBP2-02669] - Staining of paraffin-embedded Human pancreas tissue using anti-eIF2A mouse monoclonal antibody.](http://images.novusbio.com/fullsize/EIF2S1-Antibody-3H4-Immunohistochemistry-Paraffin-NBP2-02669-img0006.jpg)
![Western Blot: eIF2 alpha/EIF2S1 Antibody (PSH04-29) [NBP3-32296] - Western blot analysis of eIF2 alpha/EIF2S1 on different lysates with Rabbit anti-eIF2 alpha/EIF2S1 antibody (NBP3-32296) at 1/2,000 dilution. Lane 1: MCF7 cell lysate Lane 2: HepG2 cell lysate Lane 3: HeLa cell lysate Lane 4: COS-1 cell lysate Lane 5: A549 cell lysate Lane 6: RAW264.7 cell lysate Lane 7: C6 cell lysate Lane 8: Mouse kidney tissue lysate Lane 9: Mouse spleen tissue lysate Lane 10: Rat kidney tissue lysate Lane 11: Rat spleen tissue lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 36 kDa Observed band size: 36 kDa Exposure time: 59 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32296) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32296_rabbit-eif2-alpha-eif2s1-mab-psh04-29-27820241628526.jpg "Western Blot: eIF2 alpha/EIF2S1 Antibody (PSH04-29) [NBP3-32296] - Western blot analysis of eIF2 alpha/EIF2S1 on different lysates with Rabbit anti-eIF2 alpha/EIF2S1 antibody (NBP3-32296) at 1/2,000 dilution. Lane 1: MCF7 cell lysate Lane 2: HepG2 cell lysate Lane 3: HeLa cell lysate Lane 4: COS-1 cell lysate Lane 5: A549 cell lysate Lane 6: RAW264.7 cell lysate Lane 7: C6 cell lysate Lane 8: Mouse kidney tissue lysate Lane 9: Mouse spleen tissue lysate Lane 10: Rat kidney tissue lysate Lane 11: Rat spleen tissue lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 36 kDa Observed band size: 36 kDa Exposure time: 59 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32296) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: eIF2 alpha/EIF2S1 Antibody (PSH04-29) [NBP3-32296] - Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-eIF2 alpha/EIF2S1 antibody (NBP3-32296) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32296) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32296_rabbit-eif2-alpha-eif2s1-mab-psh04-29-29820241420926.jpg)
![Immunocytochemistry/ Immunofluorescence: eIF2 alpha/EIF2S1 Antibody (PSH04-29) [NBP3-32296] - Immunocytochemistry analysis of HepG2 cells labeling eIF2 alpha/EIF2S1 with Rabbit anti-eIF2 alpha/EIF2S1 antibody (NBP3-32296) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-eIF2 alpha/EIF2S1 antibody (NBP3-32296) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32296_rabbit-eif2-alpha-eif2s1-mab-psh04-29-29202410484850.jpg)
![Flow Cytometry: eIF2 alpha/EIF2S1 Antibody (PSH04-29) [NBP3-32296] - Flow cytometric analysis of HepG2 cells labeling eIF2 alpha/EIF2S1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32296, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32296_rabbit-eif2-alpha-eif2s1-mab-psh04-29-2920241645323.jpg)
![Western Blot: eIF2 alpha/EIF2S1 Antibody (3H12-C11) [H00001965-M01] - Analysis of EIF2S1 expression in transfected 293T cell line by EIF2S1 monoclonal antibody (M01), clone 3H12-C11.Lane 1: EIF2S1 transfected lysate(36.1 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/eIF2-alpha-EIF2S1-Antibody-3H12-C11-Western-Blot-H00001965-M01-img0019.jpg "Western Blot: eIF2 alpha/EIF2S1 Antibody (3H12-C11) [H00001965-M01] - Analysis of EIF2S1 expression in transfected 293T cell line by EIF2S1 monoclonal antibody (M01), clone 3H12-C11.Lane 1: EIF2S1 transfected lysate(36.1 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: eIF2 alpha/EIF2S1 Antibody (3H12-C11) [H00001965-M01] - Analysis of monoclonal antibody to EIF2S1 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/eIF2-alpha-EIF2S1-Antibody-3H12-C11-Immunocytochemistry-Immunofluorescence-H00001965-M01-img0014.jpg)
![Western Blot: eIF2 alpha/EIF2S1 Antibody (3H12-C11) [H00001965-M01] - Analysis of EIF2S1 over-expressed 293 cell line, cotransfected with EIF2S1 Validated Chimera RNAi ( Cat # H00001965-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with EIF2S1 monoclonal antibody (M01), clone 3H12-C11 (Cat # H00001965-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/eIF2-alpha-EIF2S1-Antibody-3H12-C11-Western-Blot-H00001965-M01-img0015.jpg)
![Western Blot: eIF2 alpha/EIF2S1 Antibody (3H12-C11) [H00001965-M01] - EIF2S1 monoclonal antibody (M01), clone 3H12-C11 Analysis of EIF2S1 expression in HeLa.](http://images.novusbio.com/fullsize/eIF2-alpha-EIF2S1-Antibody-3H12-C11-Western-Blot-H00001965-M01-img0017.jpg)
![Western Blot: eIF2 alpha/EIF2S1 Antibody (3H12-C11) [H00001965-M01] - EIF2S1 monoclonal antibody (M01), clone 3H12-C11. Analysis of EIF2S1 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/eIF2-alpha-EIF2S1-Antibody-3H12-C11-Western-Blot-H00001965-M01-img0018.jpg)
![ELISA: eIF2 alpha/EIF2S1 Antibody (3H12-C11) [H00001965-M01] - Detection limit for recombinant GST tagged EIF2S1 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/eIF2-alpha-EIF2S1-Antibody-3H12-C11-ELISA-H00001965-M01-img0020.jpg)
![Western Blot: eIF2 alpha/EIF2S1 [p Ser51] Antibody (RM298) [NBP2-61573] - HeLa cell lysate nontreated (-) or treated (+) with Calyculin A (CalA), using NBP2-61573.](http://images.novusbio.com/fullsize/eIF2-alpha-EIF2S1-p-Ser51-Antibody-RM298-Western-Blot-NBP2-61573-img0007.jpg "Western Blot: eIF2 alpha/EIF2S1 [p Ser51] Antibody (RM298) [NBP2-61573] - HeLa cell lysate nontreated (-) or treated (+) with Calyculin A (CalA), using NBP2-61573.")
![Immunohistochemistry-Paraffin: eIF2 alpha/EIF2S1 [p Ser51] Antibody (RM298) [NBP2-61573] - Human thyroid cancer tissue section using NBP2-61573.](http://images.novusbio.com/fullsize/eIF2-alpha-EIF2S1-p-Ser51-Antibody-RM298-Immunohistochemistry-Paraffin-NBP2-61573-img0005.jpg)
![Immunohistochemistry-Paraffin: eIF2 alpha/EIF2S1 [p Ser51] Antibody (RM298) [NBP2-61573] - Human breast cancer tissue section using NBP2-61573.](http://images.novusbio.com/fullsize/eIF2-alpha-EIF2S1-p-Ser51-Antibody-RM298-Immunohistochemistry-Paraffin-NBP2-61573-img0003.jpg)
![Western Blot: eIF2 alpha/EIF2S1 Antibody [NB100-2249] - Detection of Human and Mouse eIF2-alpha on HeLa whole cell lysate using NB100-2249.](http://images.novusbio.com/fullsize/eIF2-alpha-EIF2S1-Antibody-Western-Blot-NB100-2249-img0001.jpg "Western Blot: eIF2 alpha/EIF2S1 Antibody [NB100-2249] - Detection of Human and Mouse eIF2-alpha on HeLa whole cell lysate using NB100-2249.")
![Immunohistochemistry-Paraffin: eIF2 alpha/EIF2S1 Antibody [NB100-2249] - Human colon carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-eIF2alpha/EIF2S1 used at a dilution of 1:1,000 (0.2ug/ml) and 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/eIF2-alpha-EIF2S1-Antibody-Immunohistochemistry-Paraffin-NB100-2249-img0003.jpg)
![Immunohistochemistry: eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6) [NBP3-26427] - Image of eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26427_rabbit-eif2-alpha-eif2s1-p-ser-51-mab-1c6-262202414471571.jpg "Immunohistochemistry: eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6) [NBP3-26427] - Image of eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.")
![Immunohistochemistry: eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6) [NBP3-26427] - Image of eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26427_rabbit-eif2-alpha-eif2s1-p-ser-51-mab-1c6-262202415475635.jpg)
![Western Blot: eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6) [NBP3-26427] - Positive Western Blot detected in Hela whole cell lysate (treated with Calyculin A or EGF). All lanes: eIF2 alpha/EIF2S1 [p Ser51] Antibody at 1.48ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 36 KDa Observed band size: 36 KDa](http://images.novusbio.com/fullsize/nbp3-26427_rabbit-eif2-alpha-eif2s1-p-ser-51-mab-1c6-282202411442641.jpg)
![Immunocytochemistry/Immunofluorescence: eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6) [NBP3-26427] - Staining of A549 cells (treated with 100ng/ml EGF for 20min) with eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6) at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26427_rabbit-eif2-alpha-eif2s1-p-ser-51-mab-1c6-282202412493624.jpg)
![Western Blot: eIF2 alpha/EIF2S1 Antibody [NBP2-49428] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251 MGLane 4: Human plasmaLane 5: Human Liver tissueLane 6: Human Tonsil tissue](http://images.novusbio.com/fullsize/eIF2-alpha-EIF2S1-Antibody-Western-Blot-NBP2-49428-img0003.jpg "Western Blot: eIF2 alpha/EIF2S1 Antibody [NBP2-49428] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251 MGLane 4: Human plasmaLane 5: Human Liver tissueLane 6: Human Tonsil tissue")
![Immunocytochemistry/Immunofluorescence: eIF2 alpha/EIF2S1 Antibody [NBP2-49428] - Immunofluorescent staining of human cell line MCF7 shows localization to cytosol.](http://images.novusbio.com/fullsize/eIF2-alpha-EIF2S1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-49428-img0004.jpg)
![Immunohistochemistry-Paraffin: eIF2 alpha/EIF2S1 Antibody [NBP2-49428] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/eIF2-alpha-EIF2S1-Antibody-Immunohistochemistry-Paraffin-NBP2-49428-img0002.jpg)
![Western Blot: eIF2 alpha/EIF2S1 Antibody [NBP1-32289] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with eIF2 alpha antibody [N1C3] diluted at 1:5000.](http://images.novusbio.com/fullsize/eIF2-alpha-EIF2S1-Antibody-Western-Blot-NBP1-32289-img0002.jpg "Western Blot: eIF2 alpha/EIF2S1 Antibody [NBP1-32289] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with eIF2 alpha antibody [N1C3] diluted at 1:5000.")
![Immunocytochemistry/Immunofluorescence: eIF2 alpha/EIF2S1 Antibody [NBP1-32289] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: eIF2 alpha protein stained by eIF2 alpha antibody [N1C3] diluted at 1:5000. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/eIF2-alpha-EIF2S1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32289-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: eIF2 alpha/EIF2S1 Antibody [NBP1-32289] - HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: eIF2 alpha protein stained by eIF2 alpha antibody [N1C3] diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/eIF2-alpha-EIF2S1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32289-img0004.jpg)
![Immunohistochemistry: eIF2 alpha/EIF2S1 [p Ser 51] Antibody (SR1604) [NBP3-21506] - Immunohistochemical analysis of paraffin-embedded human colon cancer, using NBP3-21506.](http://images.novusbio.com/fullsize/nbp3-21506_rabbit-eif2-alpha-eif2s1-p-ser-51-mab-sr1604-5720231595524.jpg "Immunohistochemistry: eIF2 alpha/EIF2S1 [p Ser 51] Antibody (SR1604) [NBP3-21506] - Immunohistochemical analysis of paraffin-embedded human colon cancer, using NBP3-21506.")
![Western Blot: eIF2 alpha/EIF2S1 [p Ser 51] Antibody (SR1604) [NBP3-21506] - Western blot analysis using NBP3-21506 in (1)HeLa cell lysates treated with Calyculin A;(2) Untreated HeLa cell lysates.](http://images.novusbio.com/fullsize/nbp3-21506_rabbit-eif2-alpha-eif2s1-p-ser-51-mab-sr1604-6720231051267.jpg)