Home » EGLN3/PHD3 » EGLN3/PHD3 Antibodies » EGLN3/PHD3 Antibody [DyLight 350]
Novus Biologicals products are now on bio-techne.com

EGLN3/PHD3 Antibody [DyLight 350]

 
 Submit a Review

Images

 
There are currently no images for EGLN3/PHD3 Antibody (NB100-303UV).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, Simple Western, ChIP, ICC/IF, IHC, IP, WB, ChIP
Clonality
Polyclonal
Host
Rabbit
Conjugate
DyLight 350

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB100-303AF350
Alexa Fluor 405
NB100-303AF405
Alexa Fluor 488
NB100-303AF488
Alexa Fluor 532
NB100-303AF532
Alexa Fluor 594
NB100-303AF594
Alexa Fluor 647
NB100-303AF647
Alexa Fluor 700
NB100-303AF700
Alexa Fluor 750
NB100-303AF750
Allophycocyanin
NB100-303APC
Biotin
NB100-303B
CoraFluor 1
NB100-303CL1
DyLight 405
NB100-303V
DyLight 488
NB100-303G
DyLight 550
NB100-303R
DyLight 594
NB100-303DL594
DyLight 650
NB100-303C
DyLight 680
NB100-303FR
DyLight 755
NB100-303IR
FITC
NB100-303F
HRP
NB100-303H
Janelia Fluor 525
NB100-303JF525
Janelia Fluor 549
NB100-303JF549
Janelia Fluor 646
NB100-303JF646
Janelia Fluor 669
NB100-303JF669
PE
NB100-303PE
PerCP
NB100-303PCP
Unconjugated
NB100-303
mFluor Violet 450 SE
NB100-303MFV450
mFluor Violet 500 SE
NB100-303MFV500
mFluor Violet 610 SE
NB100-303MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NB100-303
BSA Free
NB100-303SS
BSA Free
NB100-303
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

EGLN3/PHD3 Antibody [DyLight 350] Summary

Immunogen
A synthetic peptide made to the C-terminus of humanPHD3/HIF Prolyl Hydroxylase 3. [LocusLink ID 112399]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EGLN3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP)
  • Chromatin Immunoprecipitation
  • Immunoblotting
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Simple Western
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Primate reactivity reported in scientific literature (PMID: 23732909). Rat reactivity reported in scientific literature (PMID: 23545307).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for EGLN3/PHD3 Antibody [DyLight 350]

  • EC 1.14.11
  • EC 1.14.11.-
  • EGL nine (C.elegans) homolog 3
  • egl nine homolog 3 (C. elegans)
  • egl nine homolog 3
  • egl nine-like protein 3 isoform
  • EGLN3
  • FLJ21620
  • HIF prolyl hydroxylase 3
  • HIFPH3 MGC125998
  • HIFPH3
  • HIF-PH3
  • HIF-prolyl hydroxylase 3
  • HPH-1
  • HPH-3
  • Hypoxia-inducible factor prolyl hydroxylase 3
  • MGC125999
  • pdh3
  • PHD3
  • Prolyl hydroxylase domain-containing protein 3

Background

HIF prolyl hydroxylase 3 (HPH-3; PHD3; EGLN3) catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins, hydroxylates HIF-1 alpha at Pro-564, and also hydroxylates HIF-2 alpha. PHD3 functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitylation complex. EGLN3 may play a role in cell growth regulation in muscle cells and in apoptosis in neuronal tissue. HIF prolyl hydroxylase 3 promotes cell death through a caspase-dependent mechanism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

EGLN1/PHD2 Antibody
NB100-137
Flow Cytometry EGLN1/PHD2 Antibody
Western Blot EGLN1/PHD2 Antibody
Immunohistochemistry EGLN1/PHD2 Antibody
(12 Reviews)
(92 Publications)
Species: Hu, Mu, Pm, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, KD, KO, Simple Western, WB
HIF-1 alpha
Antibody (H1alpha67)
NB100-105
Western Blot HIF-1 alpha Antibody (H1alpha67)
Flow Cytometry HIF-1 alpha Antibody (H1alpha67)
Flow Cytometry HIF-1 alpha Antibody (H1alpha67)
(45 Reviews)
(1043 Publications)
Species: Bv, Ca, Fe, Ma, Hu, Pm, Mu, Po, Pm, Rb, Rt, Sh, Xp
Applications: ChIP, ChIP, ELISA, Flow, GS, IA, IB, ICC/IF, IHC-FrFl, IHC, IHC-Fr, IHC-P, IP, In vitro, KD, KO, LA, PLA, Simple Western, TCS, WB
EGLN2/PHD1 Antibody
AF6394
Western Blot EGLN2/PHD1 Antibody
Immunohistochemistry EGLN2/PHD1 Antibody
Immunohistochemistry EGLN2/PHD1 Antibody
(7 Publications)
Species: Hu
Applications: IP, WB
VEGF [HRP]
DVE00
N/A VEGF [HRP]
N/A VEGF [HRP]
N/A VEGF [HRP]
(684 Publications)
Species: Hu
Applications: ELISA
Von Hippel Lindau
Antibody (OTI1E1)
NBP2-45411
Immunohistochemistry Von Hippel Lindau Antibody (OTI1E1)
Immunohistochemistry Von Hippel Lindau Antibody (OTI1E1)
Immunohistochemistry Von Hippel Lindau Antibody (OTI1E1)
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
HYPB Antibody -
Azide and BSA Free
NBP3-03807
Immunocytochemistry/ Immunofluorescence HYPB Antibody - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence HYPB Antibody - Azide and BSA Free
Western Blot HYPB Antibody - Azide and BSA Free
Species: Hu, Mu, Rt
Applications: ICC/IF, IP, WB
HIF-2 alpha/EPAS1
Antibody - BSA Free
NB100-122
Immunohistochemistry HIF-2 alpha/EPAS1 Antibody - BSA Free
Immunohistochemistry HIF-2 alpha/EPAS1 Antibody - BSA Free
Western Blot HIF-2 alpha/EPAS1 Antibody - BSA Free
(37 Reviews)
(826 Publications)
Species: Fi, Ha, Hu, Mu, Pm, Rb, Rt, Re, Sh
Applications: ChIP, Dual ISH-IHC, ELISA, Flow, GS, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, In vitro, KD, KO, PAGE, Simple Western, WB
Erythropoietin/EPO
[HRP]
MEP00B
N/A Erythropoietin/EPO [HRP]
N/A Erythropoietin/EPO [HRP]
(105 Publications)
Species: Mu
Applications: ELISA
ARNT/HIF-1 beta
Antibody (H1beta234) ...
NB100-124
Western Blot ARNT/HIF-1 beta Antibody (H1beta234) - BSA Free
Western Blot ARNT/HIF-1 beta Antibody (H1beta234) - BSA Free
Immunocytochemistry/ Immunofluorescence ARNT/HIF-1 beta Antibody (H1beta234) - BSA Free
(4 Reviews)
(106 Publications)
Species: Bv, Ma, Hu, Mu, Pm, Rt, Sh
Applications: ChIP, CHIP-SEQ, GS, IB, ICC/IF, IHC, IHC-P, IP, WB
FIH-1/HIF-1AN
Antibody
NB100-428
Western Blot FIH-1/HIF-1AN Antibody
Western Blot FIH-1/HIF-1AN Antibody
Immunohistochemistry FIH-1/HIF-1AN Antibody
(3 Reviews)
(29 Publications)
Species: Hu, Mu, Rt
Applications: EM, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD, KO, WB
Calcium-sensing
R/CaSR Antibody (5C10 ...
NB120-19347
Western Blot Calcium-sensing R/CaSR Antibody (5C10, ADD) - BSA Free
Immunocytochemistry/ Immunofluorescence Calcium-sensing R/CaSR Antibody (5C10, ADD) - BSA Free
Immunocytochemistry/ Immunofluorescence Calcium-sensing R/CaSR Antibody (5C10, ADD) - BSA Free
(1 Review)
(21 Publications)
Species: Bv, Hu, Mu, Po, Rt
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, WB
GCH1 Antibody
NBP1-84949
Immunohistochemistry-Paraffin GCH1 Antibody
Immunohistochemistry-Paraffin GCH1 Antibody
Immunohistochemistry-Paraffin GCH1 Antibody
(1 Publication)
Species: Hu
Applications: ICC/IF, IHC, IHC-P
eNOS Antibody -
BSA Free
NB300-500
Western Blot eNOS Antibody - BSA Free
Immunohistochemistry-Paraffin eNOS Antibody - BSA Free
Immunohistochemistry-Paraffin eNOS Antibody - BSA Free
(3 Reviews)
(28 Publications)
Species: Am, Bv, Ca, Dr, Hu, Mu, Po, Rt, Sh
Applications: IHC, IHC-Fr, IHC-P, WB
ALDH9A1 Antibody
NBP1-89150
Western Blot ALDH9A1 Antibody
Western Blot ALDH9A1 Antibody
Immunohistochemistry-Paraffin ALDH9A1 Antibody
(3 Publications)
Species: Hu, Rt
Applications: IHC, IHC-P, WB
ILT6/CD85e/LILRA3
Antibody (2E9)
H00011026-M01
Western Blot ILT6/CD85e/LILRA3 Antibody (2E9)
(6 Publications)
Species: Hu
Applications: ELISA, WB
Dihydrolipoamide
Dehydrogenase/DLD Ant ...
NBP1-31302
Immunocytochemistry/ Immunofluorescence Dihydrolipoamide Dehydrogenase/DLD Antibody
Western Blot Dihydrolipoamide Dehydrogenase/DLD Antibody
Western Blot Dihydrolipoamide Dehydrogenase/DLD Antibody
(1 Review)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KO, WB
Morg1 Antibody
(4E12)
H00084292-M02
Immunocytochemistry/ Immunofluorescence Morg1 Antibody (4E12)
Western Blot Morg1 Antibody (4E12)
Sandwich ELISA Morg1 Antibody (4E12)
(1 Publication)
Species: Hu, Mu
Applications: ELISA, ICC/IF, IHC, IHC-Fr, S-ELISA, WB
EGLN3/PHD3
Antibody [DyLight 350
NB100-303UV
EGLN3/PHD3 Antibody [DyLight 350]
Species: Hu, Mu, Rt, Pm
Applications: WB, Simple Western, ChIP, ICC/IF, IHC, IP, WB, ChIP

Publications for EGLN3/PHD3 Antibody (NB100-303UV) (0)

There are no publications for EGLN3/PHD3 Antibody (NB100-303UV).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for EGLN3/PHD3 Antibody (NB100-303UV) (0)

There are no reviews for EGLN3/PHD3 Antibody (NB100-303UV). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for EGLN3/PHD3 Antibody (NB100-303UV) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional EGLN3/PHD3 Products

Research Areas for EGLN3/PHD3 Antibody (NB100-303UV)

Find related products by research area.

Blogs on EGLN3/PHD3

There are no specific blogs for EGLN3/PHD3, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Western Blot: PHD3/HIF Prolyl Hydroxylase 3 Overexpression Lysate (Adult Normal) [NBL1-10156] - Left - Empty vector transfected control cell lysate (HEK293 cell lysate); Right - Over-expression Lysate for HIF Prolyl Hydroxylase 3.
EGLN3/PHD3 Overexpression Lysate
NBL1-10156

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our EGLN3/PHD3 Antibody [DyLight 350] and receive a gift card or discount.


Bioinformatics

Gene Symbol EGLN3