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EGLN3/PHD3 Antibody [PE]

 
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Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, Simple Western, ChIP, ICC/IF, IHC, IP, WB, ChIP
Clonality
Polyclonal
Host
Rabbit
Conjugate
PE

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB100-303AF350
Alexa Fluor 405
NB100-303AF405
Alexa Fluor 488
NB100-303AF488
Alexa Fluor 532
NB100-303AF532
Alexa Fluor 594
NB100-303AF594
Alexa Fluor 647
NB100-303AF647
Alexa Fluor 700
NB100-303AF700
Alexa Fluor 750
NB100-303AF750
Allophycocyanin
NB100-303APC
Biotin
NB100-303B
CoraFluor 1
NB100-303CL1
DyLight 350
NB100-303UV
DyLight 405
NB100-303V
DyLight 488
NB100-303G
DyLight 550
NB100-303R
DyLight 594
NB100-303DL594
DyLight 650
NB100-303C
DyLight 680
NB100-303FR
DyLight 755
NB100-303IR
FITC
NB100-303F
HRP
NB100-303H
Janelia Fluor 525
NB100-303JF525
Janelia Fluor 549
NB100-303JF549
Janelia Fluor 635
NB100-303JF635
Janelia Fluor 646
NB100-303JF646
Janelia Fluor 669
NB100-303JF669
PerCP
NB100-303PCP
Unconjugated
NB100-303
mFluor Violet 450 SE
NB100-303MFV450
mFluor Violet 500 SE
NB100-303MFV500
mFluor Violet 610 SE
NB100-303MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NB100-303
BSA Free
NB100-303SS
BSA Free
NB100-303
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EGLN3/PHD3 Antibody [PE] Summary

Immunogen
A synthetic peptide made to the C-terminus of humanPHD3/HIF Prolyl Hydroxylase 3. [LocusLink ID 112399]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EGLN3
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP)
  • Chromatin Immunoprecipitation
  • Immunoblotting
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Simple Western
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Primate reactivity reported in scientific literature (PMID: 23732909). Rat reactivity reported in scientific literature (PMID: 23545307).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for EGLN3/PHD3 Antibody [PE]

  • EC 1.14.11
  • EC 1.14.11.-
  • EGL nine (C.elegans) homolog 3
  • egl nine homolog 3 (C. elegans)
  • egl nine homolog 3
  • egl nine-like protein 3 isoform
  • EGLN3
  • FLJ21620
  • HIF prolyl hydroxylase 3
  • HIFPH3 MGC125998
  • HIFPH3
  • HIF-PH3
  • HIF-prolyl hydroxylase 3
  • HPH-1
  • HPH-3
  • Hypoxia-inducible factor prolyl hydroxylase 3
  • MGC125999
  • pdh3
  • PHD3
  • Prolyl hydroxylase domain-containing protein 3

Background

HIF prolyl hydroxylase 3 (HPH-3; PHD3; EGLN3) catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins, hydroxylates HIF-1 alpha at Pro-564, and also hydroxylates HIF-2 alpha. PHD3 functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitylation complex. EGLN3 may play a role in cell growth regulation in muscle cells and in apoptosis in neuronal tissue. HIF prolyl hydroxylase 3 promotes cell death through a caspase-dependent mechanism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol EGLN3