![Western Blot: EGLN3/PHD3 Antibody (JE60-82) [NBP3-32745] - Western blot analysis of EGLN3/PHD3 on different lysates with Rabbit anti-EGLN3/PHD3 antibody (NBP3-32745) at 1/1,000 dilution. Lane 1: Mouse liver tissue lysate (40 ug/Lane) Lane 2: K-562 cell lysate (20 ug/Lane) Lane 3: A375 cell lysate (20 ug/Lane) Lane 4: HEK-293 cell lysate (20 ug/Lane) Predicted band size: 27 kDa Observed band size: 30 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32745) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32745_rabbit-egln3-phd3-mab-je60-82-27820241631688.jpg "Western Blot: EGLN3/PHD3 Antibody (JE60-82) [NBP3-32745] - Western blot analysis of EGLN3/PHD3 on different lysates with Rabbit anti-EGLN3/PHD3 antibody (NBP3-32745) at 1/1,000 dilution. Lane 1: Mouse liver tissue lysate (40 ug/Lane) Lane 2: K-562 cell lysate (20 ug/Lane) Lane 3: A375 cell lysate (20 ug/Lane) Lane 4: HEK-293 cell lysate (20 ug/Lane) Predicted band size: 27 kDa Observed band size: 30 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32745) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB |
| Clone | JE60-82 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant full length protein within human EGLN3/PHD3. (Uniprot: Q9H6Z9) |
| Localization | Cytoplasm. Nucleus. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | EGLN3 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 27 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for EGLN3/PHD3 Antibody (NBP3-32745)Find related products by research area.
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| Gene Symbol | EGLN3 |
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
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