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EFHD1 Antibody - BSA Free

Images

 
Western Blot: EFHD1 Antibody [NBP1-77134] - Human spleen tissue EFHD1 antibody at (A) at 2 and (B) 4 ug/ml.
Immunocytochemistry/ Immunofluorescence: EFHD1 Antibody [NBP1-77134] - Rat spleen tissue with EFHD1 antibody at 5 ug/mL.
Western Blot: EFHD1 Antibody - BSA Free [NBP1-77134] - Western blot analysis of EFHD1 in human spleen tissue lysate with EFHD1 antibody at (A) 2 and (B) 4 u/mL.
Immunocytochemistry/ Immunofluorescence: EFHD1 Antibody - BSA Free [NBP1-77134] - Immunofluorescence of EFHD1 in Rat Spleen cells with EFHD1 antibody at 20 u/mL.
Immunocytochemistry/ Immunofluorescence: EFHD1 Antibody - BSA Free [NBP1-77134] - Immunofluorescence of EFHD1 in Rat Spleen cells with EFHD1 antibody at 20 u/mL.
Immunocytochemistry/ Immunofluorescence: EFHD1 Antibody - BSA Free [NBP1-77134] - Immunofluorescence of EFHD1 in Rat Spleen cells with EFHD1 antibody at 20 u/mL.
Immunocytochemistry/ Immunofluorescence: EFHD1 Antibody - BSA Free [NBP1-77134] - Immunofluorescence of EFHD1 in Rat Spleen cells with EFHD1 antibody at 20 u/mL.
Western Blot: EFHD1 Antibody - BSA Free [NBP1-77134] - Western blot analysis of EFHD1 in human spleen tissue lysate with EFHD1 antibody at (A) 2 and (B) 4 u/mL.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

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EFHD1 Antibody - BSA Free Summary

Immunogen
Antibody was raised against a 14 amino acid synthetic peptide near the amino terminus of human EFHD1. The immunogen is located within the first 50 amino acids of EFHD1. Amino Acid Squence: EPKPEPEPPARAPT
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EFHD1
Purity
Peptide affinity purified
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Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/mL
  • Western Blot 2-4 ug/ml
Control
Human Spleen Whole Tissue Lysate (Adult Whole Normal)
Control Peptide
EFHD1 Peptide (NBP1-77134PEP)
Publications
Read Publication using NBP1-77134.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for EFHD1 Antibody - BSA Free

  • DKFZp781H0842
  • EF-hand domain family, member D1
  • EF-hand domain-containing protein 1
  • EF-hand domain-containing protein D1
  • member D1
  • MST133
  • MSTP133
  • swiprosin-2

Background

EFHD1, also known as Swiprosin-2 or SWS2, is an EF-hand and coiled-coil-containing adaptor protein identified in a subtractive hybridization study using a neuronal cell line established from the cerebellum of an adult p53-null mouse, however further study indicated no difference in normal mice. Its mRNA is widely expressed, with its expression in brain undetectable at embryonic stages, with increasing levels from postnatal to adult development. In situ hybridization showed expression in neurons but not white matter of the cerebellum and cerebrum. EHFD1 is also highly expressed in testes, ovary, and the collecting ducts of the kidney, suggesting that in non-neuronal cells, EFHD1 may be involved in gametogenesis and water-reabsorbtion.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for EFHD1 Antibody (NBP1-77134)(1)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for EFHD1 Antibody (NBP1-77134). (Showing 1 - 1 of 1 FAQ).

  1. I have used your EFHD1 antibody (NBP1-71699) and can detect a band at approximately 150kDa. Have you detected this band as well?
    • EFHD1 protein is 239 amino acids in length with a molecular weight of 27kDa. That is the band that our antibody is recognizing. We have not observed the 150kDa band that you are referring to and cannot comment on its origin. You should observe a band at approximately 27kDa on your Western blot.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Research Areas for EFHD1 Antibody (NBP1-77134)

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Blogs on EFHD1

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Bioinformatics

Gene Symbol EFHD1
Entrez
Uniprot