EFHD1 Antibody - BSA Free Summary
Immunogen |
Antibody was raised against a 14 amino acid synthetic peptide near the amino terminus of human EFHD1. The immunogen is located within the first 50 amino acids of EFHD1. Amino Acid Squence: EPKPEPEPPARAPT |
Isotype |
IgG |
Clonality |
Polyclonal |
Host |
Rabbit |
Gene |
EFHD1 |
Purity |
Peptide affinity purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- ELISA 1:100-1:2000
- Immunocytochemistry/ Immunofluorescence 20 ug/mL
- Western Blot 2-4 ug/ml
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Control |
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Control Peptide |
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Publications |
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Packaging, Storage & Formulations
Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer |
PBS |
Preservative |
0.02% Sodium Azide |
Concentration |
1 mg/ml |
Purity |
Peptide affinity purified |
Alternate Names for EFHD1 Antibody - BSA Free
Background
EFHD1, also known as Swiprosin-2 or SWS2, is an EF-hand and coiled-coil-containing adaptor protein identified in a subtractive hybridization study using a neuronal cell line established from the cerebellum of an adult p53-null mouse, however further study indicated no difference in normal mice. Its mRNA is widely expressed, with its expression in brain undetectable at embryonic stages, with increasing levels from postnatal to adult development. In situ hybridization showed expression in neurons but not white matter of the cerebellum and cerebrum. EHFD1 is also highly expressed in testes, ovary, and the collecting ducts of the kidney, suggesting that in non-neuronal cells, EFHD1 may be involved in gametogenesis and water-reabsorbtion.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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Publications for EFHD1 Antibody (NBP1-77134)(1)
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Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
Video Protocols
FAQs for EFHD1 Antibody (NBP1-77134). (Showing 1 - 1 of 1 FAQ).
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I have used your EFHD1 antibody (NBP1-71699) and can detect a band at approximately 150kDa. Have you detected this band as well?
- EFHD1 protein is 239 amino acids in length with a molecular weight of 27kDa. That is the band that our antibody is recognizing. We have not observed the 150kDa band that you are referring to and cannot comment on its origin. You should observe a band at approximately 27kDa on your Western blot.
Control Lysate(s)
Secondary Antibodies
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Isotype Controls
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