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EFHD2 Antibody - BSA Free

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Western Blot: EFHD2 Antibody [NBP1-77135] - Analysis of EFHD2 in mouse brain tissue lysate with EFHD2 antibody at (A) 1 and (B) 2 ug/mL.
Western Blot: EFHD2 Antibody [NBP1-77135] - Analysis of EFHD2 in mouse brain tissue lysate with EFHD2 antibody at (A) 1 and (B) 2 ug/mL.
Immunocytochemistry/ Immunofluorescence: EFHD2 Antibody [NBP1-77135] - Human brain tissue with EFHD2 antibody at 20 ug/mL.
Immunohistochemistry-Paraffin: EFHD2 Antibody [NBP1-77135] - Human brain tissue with EFHD2 antibody at 5 ug/mL.
Immunohistochemistry: EFHD2 Antibody - BSA Free [NBP1-77135] - Immunohistochemistry of EFHD2 in human brain tissue with EFHD2 antibody at 5 u/mL.
Immunohistochemistry: EFHD2 Antibody - BSA Free [NBP1-77135] - Immunohistochemistry of EFHD2 in human brain tissue with EFHD2 antibody at 5 u/mL.
Immunocytochemistry/ Immunofluorescence: EFHD2 Antibody - BSA Free [NBP1-77135] - Immunofluorescence of EFHD2 in human brain tissue with EFHD2 antibody at 20 u/mL.
Western Blot: EFHD2 Antibody - BSA Free [NBP1-77135] - Western blot analysis of EFHD2 in mouse brain tissue lysate with EFHD2 antibody at (A) 1 and (B) 2 u/mL.
Western Blot: EFHD2 Antibody - BSA Free [NBP1-77135] - Western blot analysis of EFHD2 in mouse brain tissue lysate with EFHD2 antibody at (A) 1 and (B) 2 u/mL.
Immunocytochemistry/ Immunofluorescence: EFHD2 Antibody - BSA Free [NBP1-77135] - Immunofluorescence of EFHD2 in human brain tissue with EFHD2 antibody at 20 u/mL.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-77135AF350
Alexa Fluor 405
NBP1-77135AF405
Alexa Fluor 488
NBP1-77135AF488
Alexa Fluor 532
NBP1-77135AF532
Alexa Fluor 594
NBP1-77135AF594
Alexa Fluor 647
NBP1-77135AF647
Alexa Fluor 700
NBP1-77135AF700
Alexa Fluor 750
NBP1-77135AF750
Biotin
NBP1-77135B
CoraFluor 1
NBP1-77135CL1
DyLight 350
NBP1-77135UV
DyLight 405
NBP1-77135V
DyLight 488
NBP1-77135G
DyLight 550
NBP1-77135R
DyLight 594
NBP1-77135DL594
DyLight 650
NBP1-77135C
DyLight 680
NBP1-77135FR
DyLight 755
NBP1-77135IR
FITC
NBP1-77135F
HRP
NBP1-77135H
Janelia Fluor 525
NBP1-77135JF525
Janelia Fluor 549
NBP1-77135JF549
Janelia Fluor 585
NBP1-77135JF585
Janelia Fluor 635
NBP1-77135JF635
Janelia Fluor 646
NBP1-77135JF646
Janelia Fluor 669
NBP1-77135JF669
PerCP
NBP1-77135PCP
mFluor Violet 450 SE
NBP1-77135MFV450
mFluor Violet 500 SE
NBP1-77135MFV500
mFluor Violet 610 SE
NBP1-77135MFV610
View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NBP1-77135-0.025mg
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EFHD2 Antibody - BSA Free Summary

Immunogen
Antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human EFHD2. The immunogen is located within the first 50 amino acids of EFHD2. Amino Acid Squence: MEGEGGGETPEQPGLN
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EFHD2
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1-2 ug/ml
Theoretical MW
27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
EFHD2 Peptide (NBP1-77135PEP)
Reviewed Applications
Read 1 Review rated 5
using
NBP1-77135 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for EFHD2 Antibody - BSA Free

  • EF hand domain containing 2
  • EF hand domain family, member D2
  • EF-hand domain family, member D2
  • EF-hand domain-containing protein D2
  • MGC4342
  • swiprosin 1
  • swiprosin-1
  • SWS1

Background

EFHD2, also known as Swiprosin-1 or SWS1, is an EF-hand and coiled-coil-containing adaptor protein that plays a role in lymphocyte physiology. EFHD2 exhibits the highest expression in CD8+ T cells and immature B cells. It provides a membrane scaffold that is required for the Syk-, SLP-65-, and PLCgamma2-dependent B-cell receptor (BCR)-induced calcium flux. EFHD2 may also regulate BCR-induced immature and primary B-cell apoptosis. It controls spontaneous apoptosis through the regulation of BCL2L1 abundance. Also, EFHD2 plays a role as negative regulator of the canonical NF-kappaB-activating branch.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-77135
EFHD2 Antibody - BSA Free
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC

Publications for EFHD2 Antibody (NBP1-77135) (0)

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Review for EFHD2 Antibody (NBP1-77135) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: mice .

Reviews using NBP1-77135:
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WB
(1)
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Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
 WB mice 04/27/2017
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Summary

ApplicationWestern Blot
Sample TestedHippocampal lysates from p20 mice
Speciesmice

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for EFHD2 Antibody (NBP1-77135) (0)

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Secondary Antibodies

 

Isotype Controls

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Recent Reviews

5
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1
4
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1
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 Verified Customer
04/27/2017
Application: WB
Species: mice
read this review

Bioinformatics

Gene Symbol EFHD2
Entrez
Uniprot