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EED Antibody [Janelia Fluor® 669]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, ChSpecies Glossary
Applications WB, ELISA, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Janelia Fluor 669

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-81819AF350
Alexa Fluor 405
NBP2-81819AF405
Alexa Fluor 488
NBP2-81819AF488
Alexa Fluor 532
NBP2-81819AF532
Alexa Fluor 594
NBP2-81819AF594
Alexa Fluor 647
NBP2-81819AF647
Alexa Fluor 700
NBP2-81819AF700
Alexa Fluor 750
NBP2-81819AF750
Biotin
NBP2-81819B
CoraFluor 1
NBP2-81819CL1
DyLight 350
NBP2-81819UV
DyLight 405
NBP2-81819V
DyLight 488
NBP2-81819G
DyLight 550
NBP2-81819R
DyLight 594
NBP2-81819DL594
DyLight 650
NBP2-81819C
DyLight 680
NBP2-81819FR
DyLight 755
NBP2-81819IR
FITC
NBP2-81819F
HRP
NBP2-81819H
Janelia Fluor 525
NBP2-81819JF525
Janelia Fluor 549
NBP2-81819JF549
Janelia Fluor 646
NBP2-81819JF646
PerCP
NBP2-81819PCP
Unconjugated
NBP2-81819
mFluor Violet 450 SE
NBP2-81819MFV450
mFluor Violet 500 SE
NBP2-81819MFV500
mFluor Violet 610 SE
NBP2-81819MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-81819
BSA Free
NBP2-81819
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EED Antibody [Janelia Fluor® 669] Summary

Immunogen
EED antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human EED. The immunogen is located within amino acids 30 - 80 of EED. Amino Acid Squence: ESGTNTERPDTPTNTPNA
Specificity
Four alternatively spliced transcript variants have been observed.
Predicted Species
Bovine (100%), Chicken (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EED
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Peptide affinity purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for EED Antibody [Janelia Fluor® 669]

  • EED
  • embryonic ectoderm development
  • HEED
  • polycomb protein EED
  • WAIT1
  • WAIT1
  • WAIT-1
  • WD protein associating with integrin cytoplasmic tails 1

Background

EED encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein interacts with enhancer of zeste 2, the cytoplasmic tail of integrin beta7, immunodeficiency virus type 1 (HIV-1) MA protein, and histone deacetylase proteins. This protein mediates repression of gene activity through histone deacetylation, and may act as a specific regulator of integrin function. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional EED Products

Array NBP2-81819JF669

Research Areas for EED Antibody (NBP2-81819JF669)

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Blogs on EED.

Check out the latest blog posts on EED.
Microglia: pruning shears for homeostatic maintenance in the brain
By Jennifer Sokolowski, MD, PhD.Microglia play a critical role in pruning neurons and synapses during homeostatic maintenance in the adult brain.1 A recent study by Ayata et al. (2018) identified regional differe...  Read full blog post.
EZH1 has more to offer than gene repression
EZH1 is part of the Polycomb-group family of proteins, which are responsible for remodeling chromatin in genes and modulating epigenetic silencing during development.  Specifically, EZHI is a component of PRC2, or polycomb repressive complex-2.  PR...  Read full blog post.
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Bioinformatics

Gene Symbol EED