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EED Antibody (GT885)

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Western Blot: EED Antibody (GT885) [NBP3-13628] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with EED antibody [GT885] (NBP3-13628) diluted at 1:500. The ...read more
Immunocytochemistry/ Immunofluorescence: EED Antibody (GT885) [NBP3-13628] - EED antibody [GT885] detects EED protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT ...read more
Immunohistochemistry-Paraffin: EED Antibody (GT885) [NBP3-13628] - EED antibody [GT885] detects EED protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat endometrial carcinoma. ...read more
Immunohistochemistry-Paraffin: EED Antibody (GT885) [NBP3-13628] - EED antibody [GT885] detects EED protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. EED stained ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ch, Fi, RM, Xp, ZeSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
GT885
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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EED Antibody (GT885) Summary

Description
Centrifuge briefly prior to opening.
Immunogen
Recombinant protein encompassing a sequence within the center region of human EED. The exact sequence is proprietary.
Predicted Species
Chicken (99%), Xenopus (96%), Bovine (100%), Rhesus Macaque (100%), Fish (95%), Zebrafish (93%). Backed by our 100% Guarantee.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
EED
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Purity
Protein G purified

Alternate Names for EED Antibody (GT885)

  • EED
  • embryonic ectoderm development
  • HEED
  • polycomb protein EED
  • WAIT1
  • WAIT1
  • WAIT-1
  • WD protein associating with integrin cytoplasmic tails 1

Background

EED encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein interacts with enhancer of zeste 2, the cytoplasmic tail of integrin beta7, immunodeficiency virus type 1 (HIV-1) MA protein, and histone deacetylase proteins. This protein mediates repression of gene activity through histone deacetylation, and may act as a specific regulator of integrin function. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for EED Antibody (NBP3-13628) (0)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for EED Antibody (NBP3-13628) (0)

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Secondary Antibodies

 

Isotype Controls

Additional EED Products

Research Areas for EED Antibody (NBP3-13628)

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Blogs on EED.

Microglia: pruning shears for homeostatic maintenance in the brain
By Jennifer Sokolowski, MD, PhD.Microglia play a critical role in pruning neurons and synapses during homeostatic maintenance in the adult brain.1 A recent study by Ayata et al. (2018) identified regional differe...  Read full blog post.

EZH1 has more to offer than gene repression
EZH1 is part of the Polycomb-group family of proteins, which are responsible for remodeling chromatin in genes and modulating epigenetic silencing during development.  Specifically, EZHI is a component of PRC2, or polycomb repressive complex-2.  PR...  Read full blog post.

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Bioinformatics

Gene Symbol EED