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Dtk/TYRO3 Antibody [Unconjugated]

 (13 Publications)
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Tyro3/Dtk was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c, follicles of vibrissae associated with upper lip) using Goat Anti-Mouse Tyro3/Dtk Antigen Affinity-purified Polyclonal Antibody (Catalog # AF759) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counter-stained with hematoxylin (blue). Specific labeling was localized to the follicles of vibrissa. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Tyro3/Dtk was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c, follicles of vibrissae associated with upper lip) using Goat Anti-Mouse Tyro3/Dtk Antigen Affinity-purified Polyclonal Antibody ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF759
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF759-SP
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Dtk/TYRO3 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Tyro3/Dtk
Ala31-Ser418
Accession # P55144
Specificity
Detects mouse Tyro3/Dtk in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
Tyro3
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF759 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Dtk/TYRO3 Antibody [Unconjugated]

  • Brt
  • BYK
  • Dtk
  • EC 2.7.10
  • EC 2.7.10.1
  • FLJ16467
  • Rse
  • Sky
  • Tif
  • TYRO3 protein tyrosine kinase
  • Tyro3
  • Tyrosine-protein kinase byk
  • Tyrosine-protein kinase DTK
  • tyrosine-protein kinase receptor TYRO3
  • Tyrosine-protein kinase RSE
  • Tyrosine-protein kinase SKY

Background

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt) and Mer (human and mouse homologues of chicken c-Eyk) constitute a new receptor tyrosine kinase subfamily. The extracellular domain of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. All three receptors bind the vitamin K-dependent protein growth-arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. The binding affinities for Gas6 is in the order of Axl > Dtk > Mer. Gas6 binding induces tyrosine phosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis. Dtk is widely expressed during embryonic development. In adults, Dtk is predominantly expressed in neurons in restricted regions of the brain.

  1. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
  2. Crosier, K.E. and P.S Crosier (1997) Pathology 29:131.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Dtk/TYRO3 Antibody (AF759)(13)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 6 applications: Flow Cytometry, ICC, IHC, Immunoprecipitation, Neutralization, Western Blot.


Filter By Application
Flow Cytometry
(3)
ICC
(1)
IHC
(1)
Immunoprecipitation
(1)
Neutralization
(1)
Western Blot
(3)
All Applications
Filter By Species
Mouse
(9)
All Species
Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using AF759 Applications Species
H Jung, SY Lee, S Lim, HR Choi, Y Choi, M Kim, S Kim, Y Lee, KH Han, WS Chung, CH Kim Anti-inflammatory clearance of amyloid-beta by a chimeric Gas6 fusion protein Nature Medicine, 2022-08-04;0(0):. 2022-08-04 [PMID: 35927581] (IHC, Mouse) IHC Mouse
KJ Rogers, B Brunton, L Mallinger, D Bohan, KM Sevcik, J Chen, N Ruggio, W Maury IL-4/IL-13 polarization of macrophages enhances Ebola virus glycoprotein-dependent infection PLoS Negl Trop Dis, 2019-12-11;13(12):e0007819. 2019-12-11 [PMID: 31825972] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Philip L. Cohen, Wen-Hai Shao Gas6/TAM Receptors in Systemic Lupus Erythematosus Disease Markers 2019-07-09 [PMID: 31360267]
Naoya Fujino, Oliver J. Brand, David J. Morgan, Toshifumi Fujimori, Aleksander M. Grabiec, Christopher P. Jagger et al. Sensing of apoptotic cells through Axl causes lung basal cell proliferation in inflammatory diseases Journal of Experimental Medicine 2019-09-02 [PMID: 31289116]
Y Yanagihash, K Segawa, R Maeda, YI Nabeshima, S Nagata Mouse macrophages show different requirements for phosphatidylserine receptor Tim4 in efferocytosis Proc. Natl. Acad. Sci. U.S.A., 2017-08-02;0(0):. 2017-08-02 [PMID: 28768810] (Western Blot, Mouse) Western Blot Mouse
A Voronova, SA Yuzwa, BS Wang, S Zahr, C Syal, J Wang, DR Kaplan, FD Miller Migrating Interneurons Secrete Fractalkine to Promote Oligodendrocyte Formation in the Developing Mammalian Brain Neuron, 2017-05-03;94(3):500-516.e9. 2017-05-03 [PMID: 28472653] (Neutralization, Mouse) Neutralization Mouse
Lew ED, Oh J, Burrola PG et al. Differential TAM receptor-ligand-phospholipid interactions delimit differential TAM bioactivities. Elife. 10/24/2014 [PMID: 25265470]

Details:
Polarity Sensitive Indicator of Viability Flow Assay Kit used for quantifying apoptosis in adherent cells or supernatant cells from Axl_TAM TKO MEF cultures - cells stained with propidium iodide which bind dead cells and pSIVA which fluoresces when bound to PtdSer (data in Figure 4B).
Zagorska A, Traves P, Lew E, Dransfield I, Lemke G Diversification of TAM receptor tyrosine kinase function. Nat Immunol, 2014-09-07;15(10):920-8. 2014-09-07 [PMID: 25194421] (Immunoprecipitation, Mouse) Immunoprecipitation Mouse
Paolino , Magdalen, Choidas , Axel, Wallner , Stephani, Pranjic , Blanka, Uribesalgo , Iris, Loeser , Stefanie, Jamieson , Amanda M, Langdon , Wallace, Ikeda , Fumiyo, Fededa , Juan Pab, Cronin , Shane J, Nitsch , Roberto, Schultz-Fademrecht , Carsten, Eickhoff , Jan, Menninger , Sascha, Unger , Anke, Torka , Robert, Gruber , Thomas, Hinterleitner , Reinhard, Baier , Gottfrie, Wolf , Dominik, Ullrich , Axel, Klebl , Bert M, Penninger , Josef M The E3 ligase Cbl-b and TAM receptors regulate cancer metastasis via natural killer cells. Nature, 2014-02-19;507(7493):508-12. 2014-02-19 [PMID: 24553136] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Hyun-Jung Park, Ji-Yeon Baen, Ye-Ji Lee, Youn-Hee Choi, Jihee Lee Kang The TAM-family receptor Mer mediates production of HGF through the RhoA-dependent pathway in response to apoptotic cells Molecular Biology of the Cell 2012-08-15 [PMID: 22740630]
Show All 13 Publications.

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Bioinformatics

Gene Symbol Tyro3
Uniprot