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DOCK1 Antibody

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Western Blot: DOCK1 Antibody [NB100-828] - Analysis of DOCK1 in A431 lysate using this antibody at 4 ug/ ml. Primary incubated for 12 hour. Detected by western blot using chemiluminescence.
Western Blot: DOCK1 Antibody [NB100-828] - (4ug/ml) of A431 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, CaSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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DOCK1 Antibody Summary

Immunogen
Peptide with sequence C-TTRKQTSVDSGIVQ corresponding to C-Terminus according to NP_001371.1.
Predicted Species
Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
DOCK1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 3 - 4 ug/ml
Application Notes
WB: Approx. 150-200 kDa band observed in A431 lysate.
Publications
Read Publication using NB100-828.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DOCK1 Antibody

  • 180 kDa protein downstream of CRK
  • ced5
  • dedicator of cytokinesis 1
  • dedicator of cyto-kinesis 1
  • DOCK1
  • DOCK180
  • DOCK180dedicator of cytokinesis protein 1
  • DOwnstream of CrK

Background

The DOCK1 protein is involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. It also functions as a guanine nucleotide exchange factor (GEF), which activates Rac Rho small GTPases by exchanging bound GDP for free GTP. The GEF activity of the DOCK1 protein may be enhanced by ELMO1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DOCK1 Antibody (NB100-828)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol DOCK1
Entrez
Uniprot