![Immunohistochemistry-Paraffin: DNMT1 Antibody (DNMT1/2061) - Azide and BSA Free [NBP3-08482] - Formalin-fixed, paraffin-embedded human Placenta stained with DNMT1 Mouse Monoclonal Antibody (DNMT1/2061).](http://images.novusbio.com/fullsize/DNMT1-Antibody-DNMT1-2061-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-08482-img0001.jpg "Immunohistochemistry-Paraffin: DNMT1 Antibody (DNMT1/2061) - Azide and BSA Free [NBP3-08482] - Formalin-fixed, paraffin-embedded human Placenta stained with DNMT1 Mouse Monoclonal Antibody (DNMT1/2061).")
| Reactivity | HuSpecies Glossary |
| Applications | IHC |
| Clone | DNMT1/2061 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07671). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | Recombinant human DNMT1 protein fragment (around aa 767-912) (exact sequence is proprietary) (Uniprot: P26358) |
| Localization | Nuclear |
| Isotype | IgG2b Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | DNMT1 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. |
| Theoretical MW | 184 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein A or G purified |
![Immunocytochemistry E-Cadherin Antibody [Unconjugated]](https://images.novusbio.com/images2/ECadherin_AF748_Immunocytochemistry_15885.jpg)
![Immunohistochemistry E-Cadherin Antibody [Unconjugated]](https://images.novusbio.com/images2/ECadherin_AF748_Immunohistochemistry_15886.jpg)
![Immunocytochemistry E-Cadherin Antibody [Unconjugated]](https://images.novusbio.com/images2/ECadherin_AF748_Immunocytochemistry_16572.jpg)
Secondary Antibodies |
Isotype Controls |
Research Areas for DNMT1 Antibody (NBP3-08482)Find related products by research area.
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The role of DNMT3B in the co-incidence of methyltransferase and tumor suppressor expression in malignancies Epigenetics is the process of heritable change in gene activity despite alteration of the hosts DNA sequence, essentially causing a change in a phenotype without a change in the genotype of a host. To change the gene sequence without interfering w... Read full blog post. |
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The role of DNMT3A in development Epigenetics is the study of heritable change in gene activity despite alteration of the hosts DNA sequence. Change in gene activity done independently of the DNA sequence is achieved by way of histone and DNA methylation. Gene silencing in DNA ... Read full blog post. |
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Controlling Epigenetic Signaling with Dnmt1 and Dnmt3b Dnmt1 belongs to the C5-methyltransferase family that repairs cytosines in dsDNA using a nucleophilic attack mechanism. Dnmt1 is the most abundant mammalian DNA methyltransferase. It is the key methylation maintenance enzyme for both DNA replication/r... Read full blog post. |
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PCNA Antibodies: Marking Cell Proliferation & DNA Replication Proliferating Cell Nuclear Antigen (PCNA), also known as the polymerase delta auxiliary protein, is a nuclear protein essential for DNA replication as well as DNA excision and mismatch repair pathways. It has a large role in cell cycle regulation and ... Read full blog post. |
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DNMT's: An Overview of 3 DNA Methyltransferases DNA methyltransferases catalyze the transfer of the methyl group from S-andenosyl methionine (SAM) to DNA. Such methylation has wide ranging function in the cell, including organismal development and cell differentiation. In cancer, abnormal hypermeth... Read full blog post. |
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| Gene Symbol | DNMT1 |
![Immunohistochemistry-Paraffin: DNMT1 Antibody (DNMT1/2061) - Azide and BSA Free [NBP3-08482] - Formalin-fixed, paraffin-embedded human Tonsil stained with DNMT1 Mouse Monoclonal Antibody (DNMT1/2061).](http://images.novusbio.com/fullsize/DNMT1-Antibody-DNMT1-2061-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-08482-img0002.jpg "Immunohistochemistry-Paraffin: DNMT1 Antibody (DNMT1/2061) - Azide and BSA Free [NBP3-08482] - Formalin-fixed, paraffin-embedded human Tonsil stained with DNMT1 Mouse Monoclonal Antibody (DNMT1/2061).")
