| Reactivity | HuSpecies Glossary |
| Applications | AC |
| Description | A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human DNA Polymerase Kappa. Source: E. coli Amino Acid Sequence: MDSTKEKCDSYKDDLLLRMGLNDNKAGMEGLDKEKINKIIMEATKGSRFYGNELKKEKQVNQRIENMMQQKAQITSQQL Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G) This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions. |
| Source | E. coli |
| Protein/Peptide Type | Recombinant Protein Antigen |
| Gene | POLK |
| Purity | >80% by SDS-PAGE and Coomassie blue staining |
| Dilutions |
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| Application Notes | This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP2-57385. It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml. For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com |
| Theoretical MW | 27 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS and 1M Urea, pH 7.4. |
| Preservative | No Preservative |
| Purity | >80% by SDS-PAGE and Coomassie blue staining |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | POLK |
![Western Blot Androgen R/NR3C4 [p Ser213, p Ser210] Antibody (156C135.2) - BSA Free](https://images.novusbio.com/images/Androgen-R-NR3C4-p-Ser213-p-Ser210-Antibody-156C135-2-Western-Blot-NB100-56603-img0004.jpg)
![Western Blot: DNA Polymerase Kappa Antibody (6F2) [H00051426-M01] - analysis of DNA Polymerase Kappa in U87MG (lane 1), T98G (lane 2), A172 (lane 3) and normal human astrocyte (lane 4) lystes using anti-DNA Polymerase Kappa antibody. Each lane loaded with 10ug of samples. The primary antibody was used at a dilution of 1:5000 and incubated for 2 hours at room temperature in 0.1 % (w/v) BSA in PBS and 0.1% (v/v) tween-20. Image from verified customer review.](https://images.novusbio.com/images/DNA-Polymerase-Kappa-Antibody-6F2-Western-Blot-H00051426-M01-img0007.jpg "Western Blot: DNA Polymerase Kappa Antibody (6F2) [H00051426-M01] - analysis of DNA Polymerase Kappa in U87MG (lane 1), T98G (lane 2), A172 (lane 3) and normal human astrocyte (lane 4) lystes using anti-DNA Polymerase Kappa antibody. Each lane loaded with 10ug of samples. The primary antibody was used at a dilution of 1:5000 and incubated for 2 hours at room temperature in 0.1 % (w/v) BSA in PBS and 0.1% (v/v) tween-20. Image from verified customer review.")