![Western Blot: Dfna5 Antibody [NBP2-80426] - Analysis of Dfna5 on SiHa cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (NBP2-80426, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Dfna5-Antibody-Western-Blot-NBP2-80426-img0006.jpg "Western Blot: Dfna5 Antibody [NBP2-80426] - Analysis of Dfna5 on SiHa cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (NBP2-80426, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Recombinant protein within Human Dfna5 aa 1-220 / 496. (SwissProt: O60443 Human; SwissProt: Q9Z2D3 Mouse) |
| Localization | Cytosol. Plasma Membrane. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | GSDME |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 55 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH7.4), 0.2% BSA, 50% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Immunogen affinity purified |
![Cochlin [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Cochlin [Unconjugated]")
| Publications using NBP2-80426 | Applications | Species |
|---|---|---|
| Jia Y, Feng B, Ji X et al. Complement Factor H attenuates TNF-?-induced inflammation by upregulating EIF3C in rheumatoid arthritis Research Square 2023-09-19 (WB, Human) | WB | Human |
| Jia, Y;Feng, B;Ji, X;Tian, X;Zhao, L;Zhou, J;Zhang, W;Li, M;Fei, Y;Wu, X; Complement factor H attenuates TNF-?-induced inflammation by upregulating EIF3C in rheumatoid arthritis Journal of translational medicine 2023-11-23 [PMID: 37996918] | ||
| Huairui Shi, Yang Gao, Zhen Dong, Ji’e Yang, Rifeng Gao, Xiao Li, Shuqi Zhang, Leilei Ma, Xiaolei Sun, Zeng Wang, Feng Zhang, Kai Hu, Aijun Sun, Junbo Ge GSDMD-Mediated Cardiomyocyte Pyroptosis Promotes Myocardial I/R Injury Circulation Research 2021-06-15 [PMID: 34015941] |
Secondary Antibodies |
Isotype Controls |
Research Areas for Dfna5 Antibody (NBP2-80426)Find related products by research area.
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| Gene Symbol | GSDME |
![Immunohistochemistry-Paraffin: Dfna5 Antibody [NBP2-80426] - Analysis in mouse small intestine tissue using Dfna5 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature.](http://images.novusbio.com/fullsize/Dfna5-Antibody-Immunohistochemistry-Paraffin-NBP2-80426-img0005.jpg "Immunohistochemistry-Paraffin: Dfna5 Antibody [NBP2-80426] - Analysis in mouse small intestine tissue using Dfna5 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature.")
![Flow Cytometry: Dfna5 Antibody [NBP2-80426] - Analysis of Methionine Aminopeptidase 2/METAP2 was done on SiHa cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/Dfna5-Antibody-Flow-Cytometry-NBP2-80426-img0001.jpg "Flow Cytometry: Dfna5 Antibody [NBP2-80426] - Analysis of Methionine Aminopeptidase 2/METAP2 was done on SiHa cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).")
![Immunohistochemistry-Paraffin: Dfna5 Antibody [NBP2-80426] - Analysis in rat testis tissue using Dfna5 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed](http://images.novusbio.com/fullsize/Dfna5-Antibody-Immunohistochemistry-Paraffin-NBP2-80426-img0002.jpg "Immunohistochemistry-Paraffin: Dfna5 Antibody [NBP2-80426] - Analysis in rat testis tissue using Dfna5 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed")
![Immunohistochemistry-Paraffin: Dfna5 Antibody [NBP2-80426] - Analysis in human thyroid gland tissue using Dfna5 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature.](http://images.novusbio.com/fullsize/Dfna5-Antibody-Immunohistochemistry-Paraffin-NBP2-80426-img0003.jpg "Immunohistochemistry-Paraffin: Dfna5 Antibody [NBP2-80426] - Analysis in human thyroid gland tissue using Dfna5 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature.")
![Immunohistochemistry-Paraffin: Dfna5 Antibody [NBP2-80426] - Analysis in human breast tissue using Dfna5 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, wash](http://images.novusbio.com/fullsize/Dfna5-Antibody-Immunohistochemistry-Paraffin-NBP2-80426-img0004.jpg "Immunohistochemistry-Paraffin: Dfna5 Antibody [NBP2-80426] - Analysis in human breast tissue using Dfna5 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, wash")
![Immunocytochemistry Caspase-3 Antibody [Unconjugated] - Active](https://images.novusbio.com/images2/Caspase-3_AF835_Immunocytochemistry_6532.jpg)
![Immunohistochemistry Caspase-3 Antibody [Unconjugated] - Active](https://images.novusbio.com/images2/Caspase3_AF835_Immunohistochemistry_22976.jpg)
![Immunocytochemistry Caspase-3 Antibody [Unconjugated] - Active](https://images.novusbio.com/images2/Caspase-3_AF835_Immunocytochemistry_9340.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")