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DCC Antibody

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Western blot shows lysates of SH-SY5Y human neuroblastoma cell line and HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human DCC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5884) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for DCC at approximately 190 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of SH-SY5Y human neuroblastoma cell line and HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human DCC Antigen Affinity-purified Polyclonal ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price
Carrier Free
AF5884-SP
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DCC Antibody Summary

Immunogen
E. coli-derived recombinant human DCC
Ser1323-Phe1447
Accession # P43146
Specificity
Detects human DCC in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) ICAM‑1, rhICAM-2, rhICAM-3, rhMadCAM-1, rhPECAM-1, rhVCAM-1, and rhCEACAM-1 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
DCC
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product or the use of this product is covered by U.S. Patents owned by The Regents of the University of California. This product is for research use only and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain alicense to use this product for such purposes, contact The Regents of the University of California.

U.S. Patent # 5,939,271, 6,277,585, and other U.S. and international patents pending.



This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DCC Antibody

  • Colorectal cancer suppressor
  • CRC18
  • CRCR1
  • DCC
  • deleted in colorectal cancer protein
  • deleted in colorectal carcinoma
  • IGDCC1colorectal tumor suppressor
  • Immunoglobulin superfamily DCC subclass member 1
  • immunoglobulin superfamily, DCC subclass, member 1
  • netrin receptor DCC
  • Tumor suppressor protein DCC

Background

Deleted in colorectal cancer (DCC) is a 170-190 kDa transmembrane glycoprotein that contains four Ig-like domains and six fibronectin type III repeats in its extracellular domain. DCC is expressed on axons during development where its binding to Netrin-1 regulates axon migration and myelination. It inhibits apoptosis when bound by Netrin-1 and can induce apoptosis in the absence of ligand. DCC also functions as an adhesion molecule and a tumor suppressor on intestinal epithelial cells. Deficiencies in DCC function are associated with the development and metastasis of many tumors. Within aa 1323-1447 of the cytoplasmic domain, human DCC shares 98% aa sequence identity with the mouse and rat DCC.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol DCC
Uniprot