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DBF4B Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: DBF4B Antibody [NBP2-13900] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus.
Immunohistochemistry-Paraffin: DBF4B Antibody [NBP2-13900] - Staining of human rectum shows strong cytoplasmic positivity with a granular pattern in glandular cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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DBF4B Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to the amino acids: HPSQENSFAPADIPVKGPLLFPEARPWLMSARCWVRPFPFVTWGCLIPHDTTPLHEEVSPCPCLRLGYLYLLLTQSLWCRVRVPSLSTAGPIPRTSHPCTLAFPSYLNDHDLGHLCQAKPQGWNTPQPFLHCGFLA
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DBF4B
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, Fixation/Permeabilization: PFA/Triton X-100.
Control Peptide
DBF4B Protein (NBP2-13900PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for DBF4B Antibody

  • Activator of S phase kinase-like protein 1
  • activator of S-phase kinase-like protein 1
  • ASKL1CHIFB
  • ASK-like protein 1
  • chifb
  • Chiffon homolog B
  • DBF4 homolog B (S. cerevisiae)
  • Dbf4-related factor 1
  • DRF1MGC15009
  • FLJ13087
  • protein DBF4 homolog B
  • ZDBF1B
  • zinc finger, DBF-type containing 1B

Background

DBF4B, also known as Protein DBF4 homolog B, consists of four isoforms of sizes 67.2 kDa, 47.2 kDa, 54.7 kDa, and 18.4 kDa and is involved in DNA replication and cellular generation by stimulating kinase activity. The protein has not been researched in association with any diseases. The protein interacts with PTEN, APC, CDC7, MDFI, and KRTAP4-12 proteins during the early S phase of cell cycle DNA replication.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Bioinformatics

Gene Symbol DBF4B