Cytokeratin 8 Antibody (LP3K) [Unconjugated]

Images

 
Cytokeratin 8 was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human Cytokeratin 8 Monoclonal Antibody (Catalog # MAB3165) at 25 µg/mL for 3 hours at room temperature. Cells were ...read more
Cytokeratin 8 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Mouse Anti-Human Cytokeratin 8 Monoclonal Antibody (Catalog # MAB3165) at 1.7 µg/mL overnight at 4 °C. Tissue ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, ICC/IF
Clone
LP3K
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

Cytokeratin 8 Antibody (LP3K) [Unconjugated] Summary

Immunogen
SV-40 transformed human neonatal keratinocyte extract
Specificity
Detects human Cytokeratin 8.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
KRT8
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 1-25 ug/mL
  • Western Blot
Reviewed Applications
Read 2 Reviews rated 5
using
MAB3165 in the following applications:

Publications
Read Publications using
MAB3165 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cytokeratin 8 Antibody (LP3K) [Unconjugated]

  • CARD2
  • CK8
  • CK-8
  • CYK8
  • Cytokeratin 8
  • Cytokeratin-8
  • EndoA
  • K2C8
  • K8
  • keratin 8
  • keratin, type II cytoskeletal 8
  • keratin-8
  • KO
  • KRT8
  • Type-II keratin Kb8

Background

Cytokeratin 8 is one of the first cytokeratins expressed in the embryo and exists in adult tissues as a heterodimer with Cytokeratin 18. Cytokeratin 8 is primarily found in the non-squamous epithelia and is expressed in the majority of adenocarcinomas and ductal carcinomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, IHC, ICC/IF

Publications for Cytokeratin 8 Antibody (MAB3165)(4)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: Flow Cytometry, IHC.


Filter By Application
Flow Cytometry
(1)
IHC
(2)
All Applications
Filter By Species
Human
(3)
All Species
Showing Publications 1 - 4 of 4.
Publications using MAB3165 Applications Species
Van Slambrouck, J;Khan, M;Verbeken, E;Choi, S;Geudens, V;Vanluyten, C;Feys, S;Vanhulle, E;Wollants, E;Vermeire, K;De Fays, C;Aversa, L;Kaes, J;Van Raemdonck, D;Vos, R;Vanaudenaerde, B;De Hertogh, G;Wauters, E;Wauters, J;Ceulemans, LJ;Mombaerts, P; Visualising SARS-CoV-2 infection of the lung in deceased COVID-19 patients EBioMedicine 2023-05-22 [PMID: 37224768] (IHC, Human) IHC Human
D Kylies, M Zimmermann, F Haas, M Schwerk, M Kuehl, M Brehler, J Czogalla, LC Hernandez, L Konczalla, Y Okabayashi, J Menzel, I Edenhofer, S Mezher, H Aypek, B Dumoulin, H Wu, S Hofmann, O Kretz, N Wanner, NM Tomas, S Krasemann, M Glatzel, C Kuppe, R Kramann, B Banjanin, RK Schneider, C Urbschat, P Arck, N Gagliani, M van Zandvo, T Wiech, F Grahammer, PJ Sáez, MN Wong, S Bonn, TB Huber, VG Puelles Expansion-enhanced super-resolution radial fluctuations enable nanoscale molecular profiling of pathology specimens Nature nanotechnology, 2023-04-10;18(4):336-342. 2023-04-10 [PMID: 37037895] (IHC, Human) IHC Human
Khan M, Yoo SJ, Clijsters M et al. Visualizing in deceased COVID-19 patients how SARS-CoV-2 attacks the respiratory and olfactory mucosae but spares the olfactory bulb Cell 11/1/2021 [PMID: 34798069]
W Li, X Zhang, J Wang, M Li, C Cao, J Tan, D Ma, Q Gao TGF?1 in fibroblasts-derived exosomes promotes epithelial-mesenchymal transition of ovarian cancer cells Oncotarget, 2017-10-06;8(56):96035-96047. 2017-10-06 [PMID: 29221185] (Flow Cytometry, Human) Flow Cytometry Human

Reviews for Cytokeratin 8 Antibody (MAB3165) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse.

Reviews using MAB3165:
Filter by Applications
IF
(2)
All Applications
Filter by Species
Human
(1)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
IF Mouse 02/19/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 24572574
SpeciesMouse
  5
reviewed by:
Verified Customer
IF Human 02/19/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 24572574
SpeciesHuman

Product General Protocols

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WB Video Protocol

FAQs for Cytokeratin 8 Antibody (MAB3165) (0)

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Secondary Antibodies

 

Isotype Controls

Additional Cytokeratin 8 Products

Blogs on Cytokeratin 8.

Cytokeratin 18 - A Intermediate Filament Cyotskeletal Component
Keratins, also called cytokeratins, are a family of filamentous structural proteins that form the intermediate filaments within epithelial cells. Keratins are differentially expressed depending on both the epithelial cell origin and degree of differen...  Read full blog post.

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Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

 
Verified Customer
02/19/2015
Application: IF
Species: Mouse

 
Verified Customer
02/19/2015
Application: IF
Species: Human

Bioinformatics

Gene Symbol KRT8