![Knockout Validated: Cytokeratin 7 Antibody [NBP1-33104] - Wild-type (WT) and Cytokeratin 7 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 7 antibody [N1C2]. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Cytokeratin-7-Antibody-Western-Blot-NBP1-33104-img0008.jpg "Knockout Validated: Cytokeratin 7 Antibody [NBP1-33104] - Wild-type (WT) and Cytokeratin 7 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 7 antibody [N1C2]. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
| Reactivity | Hu, PmSpecies Glossary |
| Applications | WB, ICC/IF, IHC, KO |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Cytokeratin 7. The exact sequence is proprietary. |
| Localization | Cytoplasm |
| Predicted Species | Chimpanzee (98%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | KRT7 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 51 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol, 1% BSA |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
![Choline Kinase beta [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Choline Kinase beta [Unconjugated]")
Secondary Antibodies |
Isotype Controls |
Research Areas for Cytokeratin 7 Antibody (NBP1-33104)Find related products by research area.
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| Gene Symbol | KRT7 |
![Western Blot: Cytokeratin 7 Antibody [NBP1-33104] - Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE, antibody diluted at 1:10000.](http://images.novusbio.com/fullsize/Cytokeratin-7-Antibody-Western-Blot-NBP1-33104-img0006.jpg "Western Blot: Cytokeratin 7 Antibody [NBP1-33104] - Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE, antibody diluted at 1:10000.")
![Immunocytochemistry/Immunofluorescence: Cytokeratin 7 Antibody [NBP1-33104] - Analysis of paraformaldehyde-fixed HeLa, using Cytokeratin.](http://images.novusbio.com/fullsize/Cytokeratin-7-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33104-img0002.jpg "Immunocytochemistry/Immunofluorescence: Cytokeratin 7 Antibody [NBP1-33104] - Analysis of paraformaldehyde-fixed HeLa, using Cytokeratin.")
![Immunohistochemistry: Cytokeratin 7 Antibody [NBP1-33104] - Immunohistochemical characterization of cytokeratin 7 in human small cell lung cancer (SCLC) and non-small cell lung cancer (NSCLC) specimens.Sample: Paraffin-embedded human SCLC (upper panel) and NSCLC (lower panel).The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 15 mins. The section was then incubated with primary antibody overnight at 4C and detected using an HRP conjugated avidin-biotin-peroxidase Complex system. DAB was used as the chromogen and counterstained with haematoxylin.](http://images.novusbio.com/fullsize/Cytokeratin-7-Antibody-Immunohistochemistry-NBP1-33104-img0010.jpg "Immunohistochemistry: Cytokeratin 7 Antibody [NBP1-33104] - Immunohistochemical characterization of cytokeratin 7 in human small cell lung cancer (SCLC) and non-small cell lung cancer (NSCLC) specimens.Sample: Paraffin-embedded human SCLC (upper panel) and NSCLC (lower panel).The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 15 mins. The section was then incubated with primary antibody overnight at 4C and detected using an HRP conjugated avidin-biotin-peroxidase Complex system. DAB was used as the chromogen and counterstained with haematoxylin.")
![Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody [NBP1-33104] - Paraffin-embedded A549 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Cytokeratin-7-Antibody-Immunohistochemistry-Paraffin-NBP1-33104-img0004.jpg "Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody [NBP1-33104] - Paraffin-embedded A549 xenograft, using antibody at 1:500 dilution.")
![Immunohistochemistry-Frozen: Cytokeratin 7 Antibody [NBP1-33104] - Analysis of human placenta (paraformaldehyde-fixed frozen sections), using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/Cytokeratin-7-Antibody-Immunohistochemistry-Frozen-NBP1-33104-img0007.jpg "Immunohistochemistry-Frozen: Cytokeratin 7 Antibody [NBP1-33104] - Analysis of human placenta (paraformaldehyde-fixed frozen sections), using antibody at 1:200 dilution.")
![Immunohistochemistry Neprilysin/CD10 Antibody [Unconjugated]](https://images.novusbio.com/images2/Neprilysin_AF1126_Immunohistochemistry_6679.jpg)
![Western Blot Neprilysin/CD10 Antibody [Unconjugated]](https://images.novusbio.com/images/af1126_mouse-neprilysin-affinity-purified-polyclonal-ab-8220249505976.jpg)
![Western Blot Neprilysin/CD10 Antibody [Unconjugated]](https://images.novusbio.com/images/af1126_mouse-neprilysin-affinity-purified-polyclonal-ab-44202415433122.jpg)
![Immunohistochemistry CEACAM7 Antibody [Unconjugated]](https://images.novusbio.com/images2/CEACAM7_AF4478_Immunohistochemistry_20616.jpg)
![Western Blot CEACAM7 Antibody [Unconjugated]](https://images.novusbio.com/images2/CEACAM-7_AF4478_Western_Blot_5475.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")