![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (KRT18/834) - IHC-Prediluted [NBP2-48056] - Human Breast Carcinoma stained with Cytokeratin 18 Monoclonal Antibody (KRT18/834).](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-KRT18-834-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-48056-img0006.jpg "Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (KRT18/834) - IHC-Prediluted [NBP2-48056] - Human Breast Carcinoma stained with Cytokeratin 18 Monoclonal Antibody (KRT18/834).")
| Reactivity | Hu, Bv(-), Ca(-), Ha(-), Mu(-), Po(-), Rt(-), Sh(-)Species Glossary |
| Applications | IHC |
| Clone | KRT18/834 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | IHC-Prediluted |
| Description | The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining. |
| Immunogen | Recombinant full-length human Cytokeratin 18 protein (Uniprot: P05783) |
| Localization | Cytoplasmic |
| Marker | Epithelial Marker |
| Specificity | This monoclonal antibody reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It is reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | KRT18 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Theoretical MW | 45 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Purity | Protein A or G purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Cytokeratin 18 Antibody (NBP2-48056)Find related products by research area.
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Cytokeratin 18 - A Intermediate Filament Cyotskeletal Component Keratins, also called cytokeratins, are a family of filamentous structural proteins that form the intermediate filaments within epithelial cells. Keratins are differentially expressed depending on both the epithelial cell origin and degree of differen... Read full blog post. |
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| Gene Symbol | KRT18 |
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (KRT18/834) - IHC-Prediluted [NBP2-48056] - Formalin-fixed, paraffin-embedded human Thyroid Carcinoma stained with Cytokeratin 18 Antibody (KRT18/834).](http://images.novusbio.com/fullsize/Cytokeratin-18-Antibody-KRT18-834-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-48056-img0001.jpg "Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (KRT18/834) - IHC-Prediluted [NBP2-48056] - Formalin-fixed, paraffin-embedded human Thyroid Carcinoma stained with Cytokeratin 18 Antibody (KRT18/834).")
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