![Western Blot: Cyclin E1 [p Thr395] Antibody [NBP3-05378] - Western blot analysis of extracts of various cell lines, using Cyclin E1 antibody (NBP3-05378). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.](http://images.novusbio.com/fullsize/Cyclin-E1-[p-Thr395]-Antibody-Western-Blot-NBP3-05378-img0002.jpg "Western Blot: Cyclin E1 [p Thr395] Antibody [NBP3-05378] - Western blot analysis of extracts of various cell lines, using Cyclin E1 antibody (NBP3-05378). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Immunogen | A synthetic phosphorylated peptide around T395 of human Cyclin E1 (NP_001229.1). LLTPP |
| Modification | p Thr395 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | CCNE1 |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS with 50% glycerol, pH7.3. |
| Preservative | 0.02% Sodium Azide |
| Purity | Affinity purified |
![Immunohistochemistry Cyclin E2 Antibody (718916) [Unconjugated]](https://images.novusbio.com/images2/Cyclin_E2_MAB7444_Immunohistochemistry_16977.jpg)
![Immunohistochemistry Cyclin E2 Antibody (718916) [Unconjugated]](https://images.novusbio.com/images2/12177.jpg)
![Cyclin E1 [p Thr395] Antibody - BSA Free](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Cyclin E1 [p Thr395] Antibody - BSA Free")
Secondary Antibodies |
Isotype Controls |
Research Areas for Cyclin E1 Antibody (NBP3-05378)Find related products by research area.
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| Gene Symbol | CCNE1 |
![Immunocytochemistry/Immunofluorescence: Cyclin E1 [p Thr395] Antibody [NBP3-05378] - Immunofluorescence analysis of U2OS cells using Cyclin E1 Rabbit pAb (NBP3-05378) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Cyclin-E1-[p-Thr395]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05378-img0005.jpg "Immunocytochemistry/Immunofluorescence: Cyclin E1 [p Thr395] Antibody [NBP3-05378] - Immunofluorescence analysis of U2OS cells using Cyclin E1 Rabbit pAb (NBP3-05378) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.")
![Immunocytochemistry/Immunofluorescence: Cyclin E1 [p Thr395] Antibody [NBP3-05378] - Immunofluorescence analysis of C6 cells using Cyclin E1 Rabbit pAb (NBP3-05378) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Cyclin-E1-[p-Thr395]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05378-img0003.jpg "Immunocytochemistry/Immunofluorescence: Cyclin E1 [p Thr395] Antibody [NBP3-05378] - Immunofluorescence analysis of C6 cells using Cyclin E1 Rabbit pAb (NBP3-05378) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.")
![Immunocytochemistry/Immunofluorescence: Cyclin E1 [p Thr395] Antibody [NBP3-05378] - Immunofluorescence analysis of NIH/3T3 cells using Cyclin E1 Rabbit pAb (NBP3-05378) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Cyclin-E1-[p-Thr395]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05378-img0004.jpg "Immunocytochemistry/Immunofluorescence: Cyclin E1 [p Thr395] Antibody [NBP3-05378] - Immunofluorescence analysis of NIH/3T3 cells using Cyclin E1 Rabbit pAb (NBP3-05378) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.")
![Immunohistochemistry Cyclin B1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Cyclin_B1_AF6000_Immunohistochemistry_11792.jpg)
![Western Blot Cyclin B1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Cyclin_B1_AF6000_Western_Blot_9526.jpg)
![Simple Western Cyclin B1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Cyclin_B1_AF6000_Simple_Western_20094.jpg)
![Western Blot p27/Kip1 [p Thr157] Antibody](https://images.novusbio.com/images2/p27_AF1555_Western_Blot_5119.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")