![Western Blot: Cyclin E1 Antibody (CCNE1/4935R) [NBP3-07488] - Western Blot Analysis of HCT-116 cell lysate using Cyclin E1 Recombinant Rabbit Monoclonal Antibody (CCNE1/4935R).](http://images.novusbio.com/fullsize/Cyclin-E1-Antibody-CCNE1-4935R-Western-Blot-NBP3-07488-img0003.jpg "Western Blot: Cyclin E1 Antibody (CCNE1/4935R) [NBP3-07488] - Western Blot Analysis of HCT-116 cell lysate using Cyclin E1 Recombinant Rabbit Monoclonal Antibody (CCNE1/4935R).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IHC |
| Clone | CCNE1/4935R |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Description | 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08810) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant human Cyclin E1 protein fragment (around aa 10-176) (exact sequence is proprietary) (Uniprot: IDP24864) |
| Localization | Nuclear |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | CCNE1 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Theoretical MW | 53 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
![Immunohistochemistry Cyclin E2 Antibody (718916) [Unconjugated]](https://images.novusbio.com/images2/Cyclin_E2_MAB7444_Immunohistochemistry_16977.jpg)
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Secondary Antibodies |
Isotype Controls |
Research Areas for Cyclin E1 Antibody (NBP3-07488)Find related products by research area.
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| Gene Symbol | CCNE1 |
![Immunohistochemistry-Paraffin: Cyclin E1 Antibody (CCNE1/4935R) [NBP3-07488] - Formalin-fixed, paraffin-embedded human endometrium stained with Cyclin E1 Recombinant Rabbit Monoclonal Antibody (CCNE1/4935R).](http://images.novusbio.com/fullsize/Cyclin-E1-Antibody-CCNE1-4935R-Immunohistochemistry-Paraffin-NBP3-07488-img0002.jpg "Immunohistochemistry-Paraffin: Cyclin E1 Antibody (CCNE1/4935R) [NBP3-07488] - Formalin-fixed, paraffin-embedded human endometrium stained with Cyclin E1 Recombinant Rabbit Monoclonal Antibody (CCNE1/4935R).")
![Immunohistochemistry-Paraffin: Cyclin E1 Antibody (CCNE1/4935R) [NBP3-07488] - Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with Cyclin E1 Recombinant Rabbit Monoclonal Antibody (CCNE1/4935R).](http://images.novusbio.com/fullsize/Cyclin-E1-Antibody-CCNE1-4935R-Immunohistochemistry-Paraffin-NBP3-07488-img0001.jpg "Immunohistochemistry-Paraffin: Cyclin E1 Antibody (CCNE1/4935R) [NBP3-07488] - Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with Cyclin E1 Recombinant Rabbit Monoclonal Antibody (CCNE1/4935R).")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")