Western Blot: CTR2 Antibody [NBP1-05199] - Detection of CTR2 in MEF cell lysate.
Simple Western: CTR2 Antibody [NBP1-05199] - Simple Western lane view shows a specific band for CTR2 in 0.5 mg/ml of MEF (left) and Human Placenta (right) lysate. This experiment was performed under reducing conditions ...read more
This CTR2 antibody is useful for Western blot, where a band is seen at approx. 71 kDa. The theoretical molecular weight of CTR2 is 15 kDa. The difference between the expected size and the observed size in Western blot is believed to be due to a multimeric form of CTR2 (Bertinato et al).
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. See Simple Western Antibody Database for Simple Western validation: Tested in MEF and Human Placenta lysate 0.5 mg/mL, separated by Size, antibody dilution of 10 ug/mL. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
71 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.1% Sodium Azide
Concentration
0.93 mg/ml
Purity
Immunogen affinity purified
Alternate Names for CTR2 Antibody - BSA Free
Copper transporter 2
COPT2SLC13A2
CTR2Solute carrier family 31 member 2
hCTR2probable low affinity copper uptake protein 2
solute carrier family 31 (copper transporters), member 2
Background
CTR2 (copper transporter 2) belongs to copper transporter (Ctr) (TC 1.A.56) family/SLC31A subfamily and it is a second Cu permease enzyme with high similarity in sequence as well as in overall organization to CTR1 i.e. 3 putative transmembrane domains with an intramembranous MX3M motif, however, it does not have an extended N-terminal domain. CTR2 localizes to intracellular compartments, most notably the endosome or lysosome and it mediates Cu transport into the cytoplasm based on measures of elevated Cu accumulation or metal responsive reporter activation in cells in which CTR2 levels have been altered.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for CTR2 Antibody (NBP1-05199). (Showing 1 - 1 of 1 FAQs).
I have searched your website to find ctr2 antibody for my rat experiment(mainly about IHC &WB), however, I found most ctr2 antibody used for mouse and human not for rat.Could you help me to check the information on ctr2 antibody ?Thanks a lot!
We have two antibodies to CTR2 - NBP1-05199 which is validated for Western blotting using samples from human and mouse, and NBP1-85512 which is validated for IHC-P using human samples. Human and mouse CTR2 share a 77% sequence homology, however I am unable to align either of these sequence with that of the rat protein, since the rat sequence is not available on UniProt. If you have the rat sequence I would be happy to perform the alignment for you, email technical@novusbio.com. If you wish to try either of these antibodies in an untested species or application I can strongly recommend our Innovators Reward Program. To participate you simply complete an online review with an image, detailing the positive or negative results of your study, and in return you receive a discount voucher for 100% of the purchase price of the reviewed product. This allows us to gain more information on our products, and enables you to save money.
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