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CPN1 Antibody

Images

 
Western Blot: CPN1 Antibody [NBP3-32212] - Western blot analysis of CPN1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary ...read more
Immunocytochemistry/ Immunofluorescence: CPN1 Antibody [NBP3-32212] - ICC staining of CPN1 in LOVO cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

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CPN1 Antibody Summary

Immunogen
Recombinant protein within human CPN1 aa 1-200 / 458. (Uniprot: P15169)
Localization
Secreted.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CPN1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Western Blot 1:500
Theoretical MW
52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
1*TBS (pH7.4), 0.2% BSA and 50% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CPN1 Antibody

  • ACBP
  • Anaphylatoxin inactivator
  • Arginine carboxypeptidase
  • carboxypeptidase N polypeptide 1 50 kD
  • Carboxypeptidase N polypeptide 1
  • Carboxypeptidase N small subunit
  • carboxypeptidase N, polypeptide 1
  • carboxypeptidase N, polypeptide 1, 50kD
  • CPN
  • CPN1
  • CPN-1
  • CPNcarboxypeptidase N catalytic chain
  • EC 3.4.17
  • EC 3.4.17.3
  • FLJ40792
  • kininase I
  • kininase-1
  • Lysine carboxypeptidase
  • Plasma carboxypeptidase B
  • SCPN
  • SCPNcarboxypeptidase N catalytic subunit
  • Serum carboxypeptidase N

Background

Carboxypeptidase N (arginine carboxypeptidase or CPN) cleaves basic amino acid residues from the carboxy terminal of peptides and proteins. The enzyme plays a central role in regulating the biologic activity of peptides such as kinins and anaphylatoxins, and therefore is also known as kininase-1 and anaphylatoxin inactivator. CPN is a tetrameric complex consisting of two identical regulatory subunits (CPN reg) and two identical catalytic subunits (CPN cat). The two glycosylated CPN reg subunits protect the two CPN cat subunits and keep them in the circulation. CPN reg is a member of the leucinerich repeat family of proteins and the gene which encodes CPN reg maps to human chromosome 8p22-p23. CPN cat is a member of the regulatory B-type carboxypeptidase group and the gene which encodes CPN cat maps to human chromosome 10.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CPN1 Antibody (NBP3-32212) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CPN1