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Carboxypeptidase M Antibody

Images

 
Western Blot: Carboxypeptidase M Antibody [NBP1-87403] - Analysis in human cell line THP-1.
Immunohistochemistry-Paraffin: Carboxypeptidase M Antibody [NBP1-87403] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: Carboxypeptidase M Antibody [NBP1-87403] - Staining of human endometrium shows strong positivity in apical membrane in glandular cells.
Immunohistochemistry-Paraffin: Carboxypeptidase M Antibody [NBP1-87403] - Staining of human kidney shows weak membranous/ secretion positivity in cells in glomeruli.
Immunohistochemistry-Paraffin: Carboxypeptidase M Antibody [NBP1-87403] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Carboxypeptidase M Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LKTVAQNYSSVTHLHSIGKSVKGRNLWVLVVGRFPKEHRIGIPEFKYVANMHGDETVGRELLLHLIDYLVTSDGKDPEITNLINSTRIHIMPSMNPDGFEAVKKPDCYYSIGRENYNQYDLNRNFPDAFEYNNVS
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CPM
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Knockdown Validated Reactivity reported in (PMID: 31401267)
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
Carboxypeptidase M Protein (NBP1-87403PEP)
Publications
Read Publications using
NBP1-87403 in the following applications:

  • KD
    1 publication
  • WB
    2 publications

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (88%). Use in Mouse reported in scientific literature (PMID:31401267).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Carboxypeptidase M Antibody

  • Carboxypeptidase M
  • CPM
  • EC 3.4.17
  • EC 3.4.17.12

Background

The protein encoded by the Carboxypeptidase M gene is a membrane-bound arginine/lysine carboxypeptidase. Its expression is associated with monocyte to macrophage differentiation. This encoded protein contains hydrophobic regions at the amino and carboxy termini and has 6 potential asparagine-linked glycosylation sites. The active site residues of carboxypeptidases A and B are conserved in this protein. Three alternatively spliced transcript variants encoding the same protein have been described for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Carboxypeptidase M Antibody (NBP1-87403)(2)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: KD, WB.


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KD
(1)
WB
(2)
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Human
(1)
Mouse
(1)
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Bioinformatics

Gene Symbol CPM