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![Western Blot: CPN1 Antibody [NBP3-32212] - Western blot analysis of CPN1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32212, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: Raji cell lysate Lane 3: Mouse pancreas tissue lysate Predicted band size: 52 kDa Observed band size: 55 kDa](http://images.novusbio.com/fullsize/nbp3-32212_rabbit-cpn1-pab-27820241631630.jpg "Western Blot: CPN1 Antibody [NBP3-32212] - Western blot analysis of CPN1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32212, 1/500) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate Lane 2: Raji cell lysate Lane 3: Mouse pancreas tissue lysate Predicted band size: 52 kDa Observed band size: 55 kDa")
![Immunocytochemistry/ Immunofluorescence: CPN1 Antibody [NBP3-32212] - ICC staining of CPN1 in LOVO cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (NBP3-32212, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32212_rabbit-cpn1-pab-29202410334058.jpg)
![Western Blot: CPN1 Antibody [NBP1-57960] - Transfected 293T, Antibody Titration: 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/CPN1-Antibody-Western-Blot-NBP1-57960-img0001.jpg "Western Blot: CPN1 Antibody [NBP1-57960] - Transfected 293T, Antibody Titration: 0.2-1 ug/ml.")
![Immunohistochemistry-Paraffin: CPN1 Antibody [NBP1-57960] - Human Heart Tissue, antibody concentration 2.5 ug/ml.](http://images.novusbio.com/fullsize/CPN1-Antibody-Immunohistochemistry-Paraffin-NBP1-57960-img0003.jpg)
![Western Blot: CPN1 Antibody [NBP2-92524] - Analysis of extracts of various cell lines, using CPN1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 20s.](http://images.novusbio.com/fullsize/CPN1-Antibody-Western-Blot-NBP2-92524-img0001.jpg "Western Blot: CPN1 Antibody [NBP2-92524] - Analysis of extracts of various cell lines, using CPN1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 20s.")
![Western Blot: CPN1 Antibody [NBP1-58016] - Transfected 293T cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/CPN1-Antibody-Western-Blot-NBP1-58016-img0002.jpg "Western Blot: CPN1 Antibody [NBP1-58016] - Transfected 293T cell lysate, concentration 0.2-1 ug/ml.")
![Western Blot: CPN1 Overexpression Lysate (Adult Normal) [NBL1-09440] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CPN1.](http://images.novusbio.com/fullsize/CPN1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-09440-img0002.jpg "Western Blot: CPN1 Overexpression Lysate (Adult Normal) [NBL1-09440] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CPN1.")
![ELISA: Human CPN1 ELISA Kit (Colorimetric) [NBP3-27420] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-27420_human-cpn1-elisa-kit-colorimetric-11420241027322.png "ELISA: Human CPN1 ELISA Kit (Colorimetric) [NBP3-27420] - Standard Curve Reference")