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Complement Component C5aR1 Antibody (32-G1) [mFluor Violet 610 SE]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clone
32-G1
Clonality
Monoclonal
Host
Rabbit
Conjugate
mFluor Violet 610 SE

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP3-12085AF350
Alexa Fluor 405
NBP3-12085AF405
Alexa Fluor 488
NBP3-12085AF488
Alexa Fluor 532
NBP3-12085AF532
Alexa Fluor 594
NBP3-12085AF594
Alexa Fluor 647
NBP3-12085AF647
Alexa Fluor 700
NBP3-12085AF700
Alexa Fluor 750
NBP3-12085AF750
Biotin
NBP3-12085B
CoraFluor 1
NBP3-12085CL1
DyLight 350
NBP3-12085UV
DyLight 405
NBP3-12085V
DyLight 488
NBP3-12085G
DyLight 550
NBP3-12085R
DyLight 594
NBP3-12085DL594
DyLight 650
NBP3-12085C
DyLight 680
NBP3-12085FR
DyLight 755
NBP3-12085IR
FITC
NBP3-12085F
HRP
NBP3-12085H
Janelia Fluor 525
NBP3-12085JF525
Janelia Fluor 549
NBP3-12085JF549
Janelia Fluor 646
NBP3-12085JF646
Janelia Fluor 669
NBP3-12085JF669
PerCP
NBP3-12085PCP
Unconjugated
NBP3-12085-0.2mg
mFluor Violet 450 SE
NBP3-12085MFV450
mFluor Violet 500 SE
NBP3-12085MFV500
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP3-12085-0.2mg
Azide and BSA Free
NBP3-12085-0.2mg
Azide and BSA Free
NBP3-12085-0.025mg
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Complement Component C5aR1 Antibody (32-G1) [mFluor Violet 610 SE] Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
This antibody was raised by immunizing mice a synthetic Complement Component C5aR1 C-terminal phosphopeptide that contained phosphoserine instead of serine at position 334 and also at the neighboring phosphorylation sites Ser-332 and Ser-338.
Specificity
The 32-G1 antibody recognizes Complement Component C5aR1 which has its serine 334 phosphorylated. The mAb 32-G1 reacted specifically with a Complement Component C5aR1 peptide that contained phosphoserine 334 and also reacted with Complement Component C5aR1s that were isolated from C5a-stimulated, transfected RBL-Complement Component C5aR1 cells.
Isotype
IgG Kappa
Clonality
Monoclonal
Host
Rabbit
Gene
C5AR1
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes

mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Complement Component C5aR1 Antibody (32-G1) [mFluor Violet 610 SE]

  • C5a anaphylatoxin chemotactic receptor
  • C5A
  • C5aR
  • C5a-R
  • C5AR1
  • C5ARC5a anaphylatoxin receptor
  • C5R1C5a ligand
  • CD88 antigen
  • CD88
  • complement component 5 receptor 1 (C5a ligand)
  • complement component 5 receptor 1
  • complement component 5a receptor 1
  • Complement Component C5a R1
  • Complement Component C5aR1

Background

The human anaphylatoxin C5a is a 74 residue glycopeptide which is generated by proteolytic cleavage of the complement factor C5 in the course of complement activation. A variety of biological effects evoked by C5a can be demonstrated, rendering this molecule an important mediartor of inflammation, with granulocytes and macrophages as the main target cells. All cellular responses to C5a are specifically mediated by interactions with the membrane bound C5a receptor, a seven transmembrane GTP binding coupled receptor that belongs to the rhodopsin supergene family. C5a receptor 1 expression has been reported in myeloid blood cells, brain, liver, lung, spleen, heart, kidney, and intestinal tract. ESTs have been isolated from B cell/lung/testis, nerve, and placenta libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol C5AR1