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Complement C4c Antibody (99-72-18) [Janelia Fluor® 635]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IP
Clone
99-72-18
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 635

Order Details

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Complement C4c Antibody (99-72-18) [Janelia Fluor® 635] Summary

Immunogen
Synthetic peptide consisting of the last 15C-terminal residues of the C4c a4-domain (Cys-EELVYELNPLDHRGR).
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
C4B
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Complement C4c Antibody (99-72-18) [Janelia Fluor® 635]

  • basic C4
  • Basic complement C4
  • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3
  • C4B1
  • C4B12
  • C4B2
  • C4B3
  • C4FMGC164979
  • CH
  • Chido form of C4
  • CO4C4B5
  • complement C4-B
  • complement C4B1a
  • complement component 4B (Chido blood group)
  • complement component 4B
  • CPAMD3FLJ60561
  • EC 2.1.1.144
  • EC 2.7.11

Background

C4 consists of three polypeptide chains (alpha, molecular weight 95 kDa, beta, molecular weight 75 kDa and gamma, molecular weight 33 kDa) and is usually present in serum at 200-600mg/L. It is a protein of the classical complement pathway. When activated by C1s, a peptide C4a is released and the generated C4b binds C2 which is in turn cleaved by C1s to produce the classical pathway C3 convertase, namely C4b2a. C4b also has opsonin activity and binds to macrophages and neutrophils via their CR1 receptor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Complement C4c Antibody (NBP3-12091JF635) (0)

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Secondary Antibodies

 

Isotype Controls

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Array NBP3-12091JF635

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Bioinformatics

Gene Symbol C4B