![Immunohistochemistry-Frozen: Complement C3c Antibody (10-02A) [NB100-64339] - Human dermis stained with Complement C3c Antibody. IHC-Fr image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Complement-C3c-Antibody-10-02A-Immunohistochemistry-Frozen-NB100-64339-img0001.jpg "Immunohistochemistry-Frozen: Complement C3c Antibody (10-02A) [NB100-64339] - Human dermis stained with Complement C3c Antibody. IHC-Fr image submitted by a verified customer review.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, Flow, IHC |
| Clone | 013E-498.2.3 (10-02A) |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Immunogen | Human C3 from normal serum, highly purified by chromatography |
| Specificity | NB100-64339 recognizes 135 kDa complement component 3c (C3c), present in blood serum. C3c is generated over the course of complement activation, where convertase C4b2a (classical pathway) and convertase C3bBb (alternative pathway) cleave C3 to C3b and C3a. C3b is further degraded into iC3b and C3dg/C3d. iC3b is then slowly cleaved (possibly by factor I) to form C3c (beta chain + alpha' chain fragment 1 + alpha' chain fragment 2), C3dg and C3f. C3 is crucial in the induction of tolerance generated when an antigen is introduced into immunoprivileged sites and this is exploited by pathogens and cancer cells to evade the immune system by inhibiting complement activation. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | C3 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | In Western Blot binds both C3c and C3 and so is not suitable for all ELISA applications. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | Borate buffered saline (pH 8.4) |
| Preservative | 0.1% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Protein A purified |
![Simple Western Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Simple_Western_21888.jpg)
![Immunocytochemistry Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Immunocytochemistry__Immunofluorescence_17777.jpg)
![Immunohistochemistry Albumin Antibody (188835) [Unconjugated] - Serum](https://images.novusbio.com/images2/Albumin_MAB1455_Immunohistochemistry_23317.jpg)
| Publication using NB100-64339 | Applications | Species |
|---|---|---|
| Janssen BJ, Huizinga EG, Raaijmakers HC et al. Structures of complement component C3 provide insights into the function and evolution of immunity. Nature. 2005-09-01 [PMID: 16177781] |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Verified Customer |
IHC-Fr | Human | 08/06/2021 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for Complement C3c Antibody (NB100-64339)Find related products by research area.
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