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COMMD1 Antibody (OTI2E2) - Azide and BSA Free

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Western Blot: COMMD1 Antibody (OTI2E2) - Azide and BSA Free [NBP2-72418] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY COMMD1.
Immunohistochemistry: COMMD1 Antibody (OTI2E2) - Azide and BSA Free [NBP2-72418] - Analysis of Human prostate tissue.
Flow Cytometry: COMMD1 Antibody (OTI2E2) - Azide and BSA Free [NBP2-72418] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostaining by COMMD1 ...read more
Western Blot: COMMD1 Antibody (OTI2E2) - Azide and BSA Free [NBP2-72418] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: ...read more
Immunohistochemistry: COMMD1 Antibody (OTI2E2) - Azide and BSA Free [NBP2-72418] - Analysis of Adenocarcinoma of Human breast tissue.
Immunohistochemistry: COMMD1 Antibody (OTI2E2) - Azide and BSA Free [NBP2-72418] - Analysis of Adenocarcinoma of Human endometrium tissue.
Immunohistochemistry: COMMD1 Antibody (OTI2E2) - Azide and BSA Free [NBP2-72418] - Analysis of Adenocarcinoma of Human ovary tissue.
Immunohistochemistry: COMMD1 Antibody (OTI2E2) - Azide and BSA Free [NBP2-72418] - Analysis of Carcinoma of Human bladder tissue.
Immunohistochemistry: COMMD1 Antibody (OTI2E2) - Azide and BSA Free [NBP2-72418] - Analysis of Carcinoma of Human pancreas tissue.
Immunohistochemistry: COMMD1 Antibody (OTI2E2) - Azide and BSA Free [NBP2-72418] - Analysis of Carcinoma of Human prostate tissue.
Immunohistochemistry: COMMD1 Antibody (OTI2E2) - Azide and BSA Free [NBP2-72418] - Analysis of Human breast tissue.
Immunohistochemistry: COMMD1 Antibody (OTI2E2) - Azide and BSA Free [NBP2-72418] - Analysis of Human Kidney tissue.
Immunohistochemistry: COMMD1 Antibody (OTI2E2) - Azide and BSA Free [NBP2-72418] - Analysis of Human lung tissue.
Immunohistochemistry: COMMD1 Antibody (OTI2E2) - Azide and BSA Free [NBP2-72418] - Analysis of Human lymphoma tissue.
Immunohistochemistry: COMMD1 Antibody (OTI2E2) - Azide and BSA Free [NBP2-72418] - Analysis of Human pancreas tissue.
Immunohistochemistry: COMMD1 Antibody (OTI2E2) - Azide and BSA Free [NBP2-72418] - Analysis of Human tonsil tissue.
Flow Cytometry: COMMD1 Antibody (OTI2E2) - Azide and BSA Free [NBP2-72418] - Analysis of Hela cells, using COMMD1 antibody (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: COMMD1 Antibody (OTI2E2) - Azide and BSA Free [NBP2-72418] - Analysis of Jurkat cells, using COMMD1 antibody (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready
Clone
OTI2E2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

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COMMD1 Antibody (OTI2E2) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human COMMD1(NP_689729) produced in HEK293T cell.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
COMMD1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for COMMD1 Antibody (OTI2E2) - Azide and BSA Free

  • C2orf5
  • COMM domain-containing protein 1
  • COMMD1
  • copper metabolism (Murr1) domain containing 1
  • copper metabolism gene MURR1
  • MGC27155
  • MURR1
  • MURR1chromosome 2 open reading frame 5 (MURR1)
  • Protein Murr1

Background

COMMD1 is a regulator of copper homeostasis, sodium uptake, and NF-kappa-B (see MIM 164011) signaling (de Bie et al.,2005 (PubMed 16267171)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol COMMD1