Western Blot: TIP120A Antibody (5D7) [H00055832-M01] - CAND1 monoclonal antibody (M01), clone 5D7 Analysis of CAND1 expression in Hela S3 NE.
Immunocytochemistry/ Immunofluorescence: TIP120A Antibody (5D7) [H00055832-M01] - Analysis of monoclonal antibody to CAND1 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: TIP120A Antibody (5D7) [H00055832-M01] - Analysis of monoclonal antibody to CAND1 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml.
Sandwich ELISA: TIP120A Antibody (5D7) [H00055832-M01] - Detection limit for recombinant GST tagged CAND1 is approximately 0.03ng/ml as a capture antibody.
CAND1 (NP_060918, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MASASYHISNLLEKMTSSDKDFRFMATNDLMTELQKDSIKLDDDSERKVVKMILKLLEDKNGEVQNLAVKCLGPLVSKVKEYQVETIVDTLCTNMLSDKE
Specificity
CAND1 - cullin-associated and neddylation-dissociated 1
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CAND1
Purity
IgG purified
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Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA.
Publications
Read Publications using H00055832-M01 in the following applications:
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for TIP120A Antibody (5D7)
cullin-associated and neddylation-dissociated 1
Cullin-associated and neddylation-dissociated protein 1
cullin-associated NEDD8-dissociated protein 1
DKFZp434M1414
FLJ90441
KIAA0829FLJ10114
p120 CAND1
TBP interacting protein
TBP-interacting protein 120A
TBP-interacting protein of 120 kDa A
TIP120AFLJ38691
TIP120FLJ10929
Background
Enhances transcription from various types of promoters . Regulatory protein that interferes with the assembly of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex and thereby down-regulates ubiquitination of target proteins. Prevents neddylation of CUL1 by physically blocking access to the neddylation site. Disrupts interactions between CUL1 and SKP1A and between CUL1 and F-box proteins
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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