Home » Collagen VII » Collagen VII Antibodies » Collagen VII Antibody (LH7.2) [Janelia Fluor® 646]

Collagen VII Antibody (LH7.2) [Janelia Fluor® 646]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clone
LH7.2
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 646

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB100-2728AF350
Alexa Fluor 532
NB100-2728AF532
Alexa Fluor 594
NB100-2728AF594
Alexa Fluor 750
NB100-2728AF750
DyLight 594
NB100-2728DL594
Janelia Fluor 549
NB100-2728JF549
Unconjugated
NB100-2728
mFluor Violet 450 SE
NB100-2728MFV450
mFluor Violet 500 SE
NB100-2728MFV500
mFluor Violet 610 SE
NB100-2728MFV610
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Collagen VII Antibody (LH7.2) [Janelia Fluor® 646] Summary

Immunogen
Cells from a single cell suspension of epidermal cells (obtained from fresh human neonatal foreskin) were lysed with Nonidet P40 in phosphate buffered saline and the insoluble pellet was sonicated to prepare insoluble fractions.
Epitope
The epitope is on the NC1 domain.
Localization
Secreted, extracellular space, extracellular matrix, basement membrane.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
COL7A1
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Collagen VII Antibody (LH7.2) [Janelia Fluor® 646]

  • Collagen 7
  • collagen alpha-1(VII) chain
  • collagen VII, alpha-1 polypeptide
  • collagen, type VII, alpha 1
  • Collagen-7
  • EBD1
  • EBDCT
  • EBR1
  • epidermolysis bullosa, dystrophic, dominant and recessive
  • LC collagen
  • long chain collagen
  • Long-chain collagen

Background

Type VII collagen fibrils are composed of three identical alpha collagen chains. It's location is restricted to the basement zone beneath stratified squamous epithelia. It functions as an anchoring fibril between the external epithelia and the underlying stroma. Mutations in the collagen V11 gene are associated with all forms of dystrophic epidermolysis bullosa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol COL7A1