Collagen VII Antibody (D5)

Images

 
Western Blot: Collagen VII Antibody (D5) [NBP3-41723] - Human Serum; Primary Ab: 3ug/ml NBP3-41723; Second Ab: 0.2ug/mL HRP-conjugated Goat Anti-Mouse IgG Polyclonal Antibody

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
D5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

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Collagen VII Antibody (D5) Summary

Immunogen
Immunized with recombinant Human Collagen VII (Uniprot#: Q02388; Pro190-Asp472 (Uniprot#: Q02388)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
COL7A1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 5-20ug/mL
  • Immunohistochemistry 5-20ug/mL
  • Western Blot 0.01-3ug/mL

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.01M PBS, pH7.4, 50% glycerol
Preservative
0.05% Proclin 300
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for Collagen VII Antibody (D5)

  • Collagen 7
  • collagen alpha-1(VII) chain
  • collagen VII, alpha-1 polypeptide
  • collagen, type VII, alpha 1
  • Collagen-7
  • EBD1
  • EBDCT
  • EBR1
  • epidermolysis bullosa, dystrophic, dominant and recessive
  • LC collagen
  • long chain collagen
  • Long-chain collagen

Background

Type VII collagen fibrils are composed of three identical alpha collagen chains. It's location is restricted to the basement zone beneath stratified squamous epithelia. It functions as an anchoring fibril between the external epithelia and the underlying stroma. Mutations in the collagen V11 gene are associated with all forms of dystrophic epidermolysis bullosa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol COL7A1