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Porcine Coagulation Factor II/Thrombin ELISA Kit (Colorimetric)

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ELISA: Coagulation Factor II/Thrombin ELISA Kit [Biotin] [KA1407] - The curve is provided for illustration only. A standard curve should be generated each time the assay is performed.
ELISA: Coagulation Factor II/Thrombin ELISA Kit [Biotin] [KA1407] - The curve is provided for illustration only. A standard curve should be generated each time the assay is performed.

Product Details

Summary
Reactivity PoSpecies Glossary
Applications ELISA, NULL
Suitable Sample Type
Cell Culture Supernatant, Cell Lysate, Plasma, Serum
Standard Curve Range
0.391 to 100 ng/mL
Sensitivity
0.24 ng/mL

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Porcine Coagulation Factor II/Thrombin ELISA Kit (Colorimetric) Summary

Description
F2 (Pig) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of pig F2.
Standard Curve Range
0.391 to 100 ng/mL
Sensitivity
0.24 ng/mL
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 7.4%
Intra-Assay
CV% < 4.8%
Spike Recovery
96%
Sample Volume
50 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
F2

Applications/Dilutions

Dilutions
  • ELISA
  • Quantification
Publications
Read Publication using
KA1407 in the following applications:

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Swine Prothrombin Antibody (100x): A 100-fold concentratedbiotinylated polyclonal antibody against swine prothrombin.
  2. Chromogen Substrate: A ready-to-use stabilized peroxidase chromogen substratetetramethylbenzidine.
  3. EIA Diluent Concentrate (10x): A 10-fold concentrated buffered protein base.
  4. Sealing Tapes: Pressure-sensitive sealing tapes that can be cut to fit the format ofthe individual assay.
  5. Stop Solution: A 0.5 N hydrochloric acid to stop the chromogen substrate reaction.
  6. Streptavidin-Peroxidase Conjugate (SP Conjugate): A 100-fold concentrate.
  7. Swine Prothrombin Microplate: A 96-well polystyrene microplate coated with amonoclonal antibody against swine prothrombin.
  8. Swine Prothrombin Standard: Swine prothrombin in a buffered protein base,lyophilized.
  9. Wash Buffer Concentrate (20x): A 20-fold concentrated buffered surfactant.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Porcine Coagulation Factor II/Thrombin ELISA Kit (Colorimetric)

  • coagulation factor II (thrombin) receptor-like 2
  • Coagulation factor II receptor-like 2 (protease-actovated receptor 3)
  • Coagulation factor II receptor-like 2
  • Coagulation Factor II
  • F2
  • PAR-3
  • PAR3proteinase-activated receptor 3
  • protease-activated receptor 3
  • proteinase-activated receptor-3
  • PT
  • serine protease
  • Thrombin receptor-like 2
  • Thrombin

Background

Proteinase-activated receptor 3 (PAR3) is a member of the Proteinase-Activated Receptor subfamily. The enzyme thrombin is involved in the activation of platelets, leukocytes, endothelial cells, and mesenchymal cells at sites of vascular injury. These cellular responses are triggered through proteolytic activation of PAR3 and other receptors by thrombin. It is believed that PAR3 functions as a cofactor for the cleavage and activation of PAR4. PAR3 expression has been documented in blood, bone marrow, lung, spleen, and vessel. ESTs have been isolated from head/neck, skeletal muscle, and skin libraries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Publications for Coagulation Factor II/Thrombin ELISA Kit (KA1407)(1)

We have publications tested in 1 confirmed species: Porcine.

We have publications tested in 1 application: ELISA.


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Product General Protocols

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FAQs for Coagulation Factor II/Thrombin ELISA Kit (KA1407). (Showing 1 - 1 of 1 FAQ).

  1. Do you have neutralizing antibodies for anti-mouse Thrombin?
    • We currently have 1 neutralizing Thrombin antibody (NBP1-95029), however it is an anti-human antibody and has only been validated in human samples. If you would like to test this antibody in mouse samples, you may be interested in participating in our Innovators Reward Program.

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Product PDFs

  F2 (Pig) ELISA Kit
  PDF Datasheet
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Bioinformatics

Gene Symbol F2