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Prothrombin Antibody

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721_B tissue lysate at a concentration of 1ug/ml.
Immunohistochemistry: Coagulation Factor II/Thrombin Antibody [NBP1-58268] - Analysis of human liver after heat-induced Antigen retrieval. Antibody concentration 5 ug/ml.
Immunohistochemistry-Paraffin: Coagulation Factor II/Thrombin Antibody [NBP1-58268] - Human Placenta Tissue
Immunohistochemistry-Paraffin: Coagulation Factor II/Thrombin Antibody [NBP1-58268] - Human Placenta Tissue, 5.0ug/ml.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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Prothrombin Antibody Summary

Description
The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.
Immunogen
Synthetic peptides corresponding to F2(coagulation factor II (thrombin)) The peptide sequence was selected from the middle region of F2 (NP_000497). Peptide sequence ISDRWVLTAAHCLLYPPWDKNFTENDLLVRIGKHSRTRYERNIEKISMLE. The peptide sequence for this immunogen was taken from within the described region.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
F2
Purity
Affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1.0 ug/ml
Theoretical MW
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-58268 in the following applications:

Publications
Read Publications using
NBP1-58268 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 30846818).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 2% Sucrose
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Affinity purified

Alternate Names for Prothrombin Antibody

  • coagulation factor II (thrombin)
  • Coagulation factor II
  • EC 3.4.21
  • EC 3.4.21.5
  • prothrombin B-chain
  • prothrombin
  • PT
  • serine protease

Background

Coagulation factor II is proteolytically cleaved to form thrombin in the first step of the coagulation cascade which ultimately results in the stemming of blood loss. F2 also plays a role in maintaining vascular integrity during development and postnatal life.Coagulation factor II is proteolytically cleaved to form thrombin in the first step of the coagulation cascade which ultimately results in the stemming of blood loss. F2 also plays a role in maintaining vascular integrity during development and postnatal life. Mutations in F2 leads to various forms of thrombosis and dysprothrombinemia. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Coagulation Factor II/Thrombin Antibody (NBP1-58268)(3)

Review for Coagulation Factor II/Thrombin Antibody (NBP1-58268) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Equine.

Reviews using NBP1-58268:
Filter by Applications
IHC-P
(1)
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Filter by Species
Equine
(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin Prothrombin NBP1-58268
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5
reviewed by:
Claudia Klein
IHC-P Equine 07/14/2017
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedPlacental tissue
SpeciesEquine

Comments

CommentsHeat mediated antigen retrieval (sodium-citrate based buffer) for 10 min.

Product General Protocols

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WB Video Protocol

FAQs for Coagulation Factor II/Thrombin Antibody (NBP1-58268) (0)

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Secondary Antibodies

 

Isotype Controls

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Research Areas for Coagulation Factor II/Thrombin Antibody (NBP1-58268)

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Blogs on Prothrombin.

Factor VIII - a key factor in the clotting process
Hemostasis, or blood clotting, follows tissue injury and involves the deployment of essential plasma procoagulants (such as prothrombin, and Factors X, IX, V, and VIII) that trigger the blood coagulation cascade. This cascade leads to the formation...  Read full blog post.

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Claudia Klein
07/14/2017
Application: IHC-P
Species: Equine

Bioinformatics

Gene Symbol F2
Entrez
Uniprot